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• W2171540322 endingPage "e22887" @default.
• W2171540322 startingPage "e22887" @default.
• W2171540322 abstract "Human mesenchymal stem cells (hMSCs) are multipotent cells capable of differentiating into a variety of mature cell types, including osteoblasts, adipocytes and chondrocytes. It has previously been shown that, when expanded in medium supplemented with fibroblast growth factor-2 (FGF-2), hMSCs show enhanced chondrogenesis (CG). Previous work concluded that the enhancement of CG could be attributed to the selection of a cell subpopulation with inherent chondrogenic potential. In this study, we show that FGF-2 pretreatment actually primed hMSCs to undergo enhanced CG by increasing basal Sox9 protein levels. Our results show that Sox9 protein levels were elevated within 30 minutes of exposure to FGF-2 and progressively increased with longer exposures. Further, we show using flow cytometry that FGF-2 increased Sox9 protein levels per cell in proliferating and non-proliferating hMSCs, strongly suggesting that FGF-2 primes hMSCs for subsequent CG by regulating Sox9. Indeed, when hMSCs were exposed to FGF-2 for 2 hours and subsequently differentiated into the chondrogenic lineage using pellet culture, phosphorylated-Sox9 (pSox9) protein levels became elevated and ultimately resulted in an enhancement of CG. However, small interfering RNA (siRNA)-mediated knockdown of Sox9 during hMSC expansion was unable to negate the prochondrogenic effects of FGF-2, suggesting that the FGF-2-mediated enhancement of hMSC CG is only partly regulated through Sox9. Our findings provide new insights into the mechanism by which FGF-2 regulates predifferentiation hMSCs to undergo enhanced CG." @default.
• W2171540322 created "2016-06-24" @default.
• W2171540322 creator A5030866046 @default.
• W2171540322 creator A5080304930 @default.
• W2171540322 date "2011-07-27" @default.
• W2171540322 modified "2023-09-26" @default.
• W2171540322 title "Fibroblast Growth Factor-2 Primes Human Mesenchymal Stem Cells for Enhanced Chondrogenesis" @default.
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• W2171540322 doi "https://doi.org/10.1371/journal.pone.0022887" @default.
• W2171540322 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3144950" @default.
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• W2171540322 hasPublicationYear "2011" @default.
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