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- W2171567007 abstract "The purpose of this study was to determine the response of antibiotics and antibiotic resistance genes (ARG) to manure management. A pilot field study was conducted using horse manure containing no antibiotics, into which chlortetracycline, tylosin, and monensin were spiked and compared to un-spiked controls. Subsequently, a large-scale field study was conducted comparing manure from a dairy with minimal use of antibiotics and a feedlot with regular subtherapeutic use of antibiotics. The manures were subjected to high-level (HL) (amending, watering, and turning) and low-level (LL) (no amending, watering, or turning) management and were monitored for antibiotic concentrations and levels of tetracycline ARG (tet(W) and tet(O)) using quantitative real-time polymerase chain reaction. All three antibiotics in the pilot study dissipated rapidly, with half-lives ranging from 6-28 days. Levels of tet(W) were significantly higher after 141 days of treatment, but levels of tet(O) were significantly lower in all treatments. In the large-scale study, tetracycline, oxytetracycline, and chlortetracycline, and the ARG tet(W) and tet(O) were present at higher initial concentrations in the feedlot manure. After six months of feedlot manure treatment, tet(W) and tet(O) had decreased significantly and antibiotic concentrations fell below 10ppb, with half-lives ranging from 14-32 days. Dairy manure treatment required only four months for similar results." @default.
- W2171567007 created "2016-06-24" @default.
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- W2171567007 date "2007-01-01" @default.
- W2171567007 modified "2023-09-26" @default.
- W2171567007 title "Response of Antibiotics and Antibiotic Resistance Genes (ARG) in High-Level and Low-Level Managed Manures" @default.
- W2171567007 hasPublicationYear "2007" @default.
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