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• W2171569887 abstract "Article4 May 1999free access Two pacemaker channels from human heart with profoundly different activation kinetics Andreas Ludwig Andreas Ludwig Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Xiangang Zong Xiangang Zong Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Juliane Stieber Juliane Stieber Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Roger Hullin Roger Hullin Innere Medizin I, Universitätsklinik Großhadern, Marchioninistrasse 15, 81377 München, Germany Search for more papers by this author Franz Hofmann Franz Hofmann Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Martin Biel Corresponding Author Martin Biel Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Andreas Ludwig Andreas Ludwig Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Xiangang Zong Xiangang Zong Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Juliane Stieber Juliane Stieber Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Roger Hullin Roger Hullin Innere Medizin I, Universitätsklinik Großhadern, Marchioninistrasse 15, 81377 München, Germany Search for more papers by this author Franz Hofmann Franz Hofmann Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Martin Biel Corresponding Author Martin Biel Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany Search for more papers by this author Author Information Andreas Ludwig1,‡, Xiangang Zong1,‡, Juliane Stieber1, Roger Hullin2, Franz Hofmann1 and Martin Biel 1 1Institut für Pharmakologie und Toxikologie der Technischen Universität München, Biedersteiner Straße 29, 80802 München, Germany 2Innere Medizin I, Universitätsklinik Großhadern, Marchioninistrasse 15, 81377 München, Germany ‡A.Ludwig and X.Zong contributed equally to this work *Corresponding author. E-mail: [email protected] The EMBO Journal (1999)18:2323-2329https://doi.org/10.1093/emboj/18.9.2323 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential. The hyperpolarization-activated cation current (If) forms an important part of the pacemaker depolarization and consists of two kinetic components (fast and slow). Recently, three full-length cDNAs encoding hyperpolarization-activated and cyclic nucleotide-gated cation channels (HCN1–3) have been cloned from mouse brain. To elucidate the molecular identity of cardiac pacemaker channels, we screened a human heart cDNA library using a highly conserved neuronal HCN channel segment and identified two cDNAs encoding HCN channels. The hHCN2 cDNA codes for a protein of 889 amino acids. The HCN2 gene is localized on human chromosome 19p13.3 and contains eight exons spanning ∼27 kb. The second cDNA, designated hHCN4, codes for a protein of 1203 amino acids. Northern blot and PCR analyses showed that both hHCN2 and hHCN4 are expressed in heart ventricle and atrium. When expressed in HEK 293 cells, either cDNA gives rise to hyperpolarization-activated cation currents with the hallmark features of native If. hHCN2 and hHCN4 currents differ profoundly from each other in their activation kinetics, being fast and slow, respectively. We thus conclude that hHCN2 and hHCN4 may underlie the fast and slow component of cardiac If, respectively. Introduction Cardiac pacemaking determines heart rate and rhythm and is generated by the slow membrane-depolarization phase occurring between action potentials (DiFrancesco, 1993; Irisawa et al., 1993). An important part of the ionic conductance underlying cardiac pacemaker depolarization was identified in the late 1970s and early 1980s (Brown et al., 1977; Yanagihara and Irisawa, 1980; DiFrancesco, 1981) and termed If (f for funny) or synonymously Ih (h for hyperpolarization activated). If is activated by membrane hyperpolarization and is carried by both Na+ and K+. Elevation of intracellular cAMP levels shifts the voltage dependence of If in the positive direction resulting in increased inward current at a fixed negative membrane potential. This is an important mechanism responsible for the acceleration of the heart rate in response to sympathetic stimulation (Brown et al., 1979). Muscarinic stimulation slows the heart rate, in part due to a decrease in cAMP level and a resulting reduction of the If current (DiFrancesco and Mangoni, 1994; Wickman et al., 1998). cAMP regulates the current by direct binding to the channel (DiFrancesco and Tortora, 1991). An If current has also been detected in a variety of neuronal cells (Pape, 1996; Lüthi and McCormick, 1998). In the brain, a major function of the current is to control the rate of rhythmic oscillations of single neurons and neuronal networks (neuronal pacemaking). Recently, three full-length cDNAs encoding hyperpolarization-activated cation channels (HAC1, HAC2 and HAC3; Ludwig et al., 1998; corresponding to mBCNG-2, mBCNG-1 and mBCNG-4; Santoro et al., 1998) have been cloned from mouse brain. In addition, a partial sequence representing a putative fourth member of the gene family was identified from mouse brain (mBCNG-3; Santoro et al., 1998). To avoid misunderstandings due to different naming of the same proteins, in this report we will use the recently proposed consensus nomenclature for cloned If channels (Clapham, 1998; Biel et al., 1999a). According to this nomenclature, If channels are designated as hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. The family members are named as follows: HCN1 corresponds to HAC2 (mBCNG-1), HCN2 corresponds to HAC1 (mBCNG-2), HCN3 corresponds to HAC3 (mBCNG-4). The primary sequences of HCN1–3 have an overall identity of ∼60%, indicating that all three channels derive from a common evolutionary ancestor. HCN channels belong to the superfamily of voltage-gated cation channels characterized by the presence of six transmembrane helices (S1–S6) and an ion-conducting P region between the fifth and sixth segment. In addition, HCN channels, like the cyclic nucleotide-gated (CNG) channels, contain a cyclic nucleotide-binding domain (CNBD) in the C-terminus conferring channel modulation by direct interaction with cAMP or cGMP (for recent reviews see Zagotta and Siegelbaum, 1996; Clapham, 1998; Biel et al., 1999a,b). In contrast to the central nervous system, there is only limited information available on the types of HCN channels underlying cardiac If currents. Northern blot and in situ hybridization experiments indicated that a transcript corresponding to HCN2 is present in mouse heart (Ludwig et al., 1998). However, it is not known whether the cardiac HCN2 isoform is identical to its neural counterpart, or whether other types of HCN channels are expressed in heart. In this paper, we report the cloning of two full-length cDNAs encoding HCN channels from human heart tissue. When expressed in human embryonic kidney (HEK) 293 cells both cDNAs are translated into functional hyperpolarization-activated cation channels with distinct properties. Results Cloning of two cardiac pacemaker channels In an attempt to identify the molecular identity of the cardiac If channel, we screened a cDNA library prepared from the atrioventricular node region of human heart with a highly conserved portion of the mouse brain HCN1 channel encoding the transmembrane segments S1–S6 and the CNBD (Ludwig et al., 1998). The cDNA clones obtained fell into two classes defined as hHCN2 and hHCN4. The open reading frames (ORFs) of the hHCN2 and hHCN4 cDNAs predict proteins of 889 and 1203 amino acids, respectively. Both proteins contain six putative transmembrane segments, a pore region and a CNBD (Figure 1A) characterizing them as members of the family of HCN channels. The overall sequence identity between hHCN2 and hHCN4 is ∼70%. The homology is highest in the transmembrane region and the CNBD with a sequence identity of 90%. The sequences of the putative pore regions are identical between hHCN2 and hHCN4 except for one conservative substitution. In contrast, both channels diverge markedly from each other in their cytoplasmic N- and C-termini. Notably, the C-terminus of hHCN4 is ∼250 amino acids longer than that of hHCN2. An interesting feature of the two cDNAs is the extraordinarily high GC content at the 5′ end of the translated sequence (87% for hHCN2 and 77% for hHCN4 over the first 500 nucleotides); this is reflected in the high occurrence of proline and glycine amino acid residues in either N-terminus. Figure 1.Primary structure of the human HCN2 and HCN4 channels and organization of the HCN2 gene. (A) Sequence alignment of hHCN2 and hHCN4. The six putative transmembrane segments (S1–S6), the pore region and the CNBD are underlined. The pore helix (PoreH) and the selectivity filter (SF) are delineated according to the Streptomyces lividans potassium channel crystal structure (Doyle et al., 1998). Exon–intron splicing sites of hHCN2 are marked by filled triangles. (B) Organization of the hHCN2 gene (upper) and corresponding distribution of exons in the domain structure of hHCN2 (lower). Upper: exons 1–8 are depicted by black boxes, the start and stop codons are indicated. Lower: transmembrane segments S1–S6 are numbered 1–6, P indicates the pore region. Download figure Download PowerPoint Genomic structure of the human HCN2 gene A search of the DDBJ/EMBL/GenBank database with the hHCN2 sequence identified the cosmid clones F18382 and R33683, which contained most of the human HCN2 gene. These cosmids, which have been localized to chromsome 19p13.3, belong to a contig constructed as part of an ongoing project to map and sequence the entire human chromosome 19 (Ashworth et al., 1995). However, the two cosmids did not cover the hHCN2 gene completely. To close the gap between the cosmids we amplified the intervening genomic sequence by PCR. The amplicon had a length of 1.2 kb and contained exon 5 of hHCN2. An additional sequence gap roughly in the middle of cosmid F18382 was reported to be unsequencable, i.e. recalcitrant to many sequencing chemistries. We subcloned and analyzed the corresponding segment, it contained 57 bp colinear to nucleotides 99–155 of the hHCN2 cDNA, demonstrating that the reported gap contains part of exon 1. The complete genomic structure of hHCN2 comprises eight exons spanning ∼27 kb (Figure 1B). The sizes of introns ranges from ∼13 kb (intron 1) to ∼370 bp (intron 6). Tissue distribution of hHCN2 and hHCN4 mRNA Northern blot analysis demonstrated that both hHCN2 (Figure 2A) and hHCN4 mRNA (Figure 2B) are expressed in human heart. hHCN2 was also abundant in brain, whereas only a weak signal on longer exposure times was detected for hHCN4 in this tissue. The length of the hHCN2 and hHCN4 transcripts are 3.4 and 7.5 kb, which is consistent with the length of the cloned cDNAs. The expression of the two HCN channels in human cardiac tissues was further examined by RT–PCR with two primer pairs amplifying specifically hHCN2 and hHCN4, respectively. RT–PCR conditions were chosen that should be able to differentiate semiquantitatively a difference in mRNA expression levels. To prove this and to compare the expression of the two HCN channels with that of a well-known cardiac ion channel, we used a primer pair specific for human GIRK4 under the same conditions. GIRK4 is a subunit of the cardiac acetylcholine-activated potassium channel (IKACh) and was shown to be expressed predominantly in the heart atrium and only marginally in the ventricle (Krapivinsky et al., 1995). Both hHCN2- and hHCN4-specific products were easily amplified from ventricular and atrial mRNA. The GIRK4 product was also generated readily from atrial mRNA, but only in very low amounts from ventricular mRNA indicating the validity of the PCR assay. Taken together these results suggest that hHCN2 and hHCN4 mRNAs are widespread throughout the human heart ventricle and atrium. Figure 2.Expression of HCN channel mRNAs. (A, B) Northern blot analysis of mRNA from human heart (He), brain (Br), placenta (Pl), lung (Lu), liver (Li), skeletal muscle (Sk), kidney (Ki) and pancreas (Pa). The blot was labeled with a probe specific for hHCN2 (A) and hHCN4 (B) and phosphorimaged for 16 h. The size of the transcripts is indicated in kilobases. (C) RT–PCR analysis. RT–PCRs with human heart ventricle (V), atrium (A) or without mRNA (C) as template was performed with primer pairs specific for hHCN2, hHCN4 or GIRK4. The sizes of amplicons were 230, 233 and 335 bp for HCN2, HCN4 and GIRK4, respectively. Download figure Download PowerPoint Electrophysiological characterization of the expressed hHCN2 and hHCN4 channel For a functional characterization of the two channels, HEK 293 cells were transfected with expression vectors carrying the full-length coding sequence of hHCN2 and hHCN4, respectively. In whole-cell voltage–clamp mode, hyperpolarizing voltage steps negative to −60 mV induced inward currents in hHCN2- (Figure 3A) and hHCN4- (Figure 3B) expressing cells. No hyperpolarization-activated current was observed in cells transfected with empty expression vector (not shown). The peak tail current amplitudes of hHCN2 and hHCN4 showed a sigmoidal dependence on the test voltage (Figure 3C). The membrane potential of half-maximal activation (V1/2) obtained by fits to Boltzmann equations was −97 ± 1 mV (n = 11) for the hHCN2 and −109 ± 1 mV (n = 20) for the hHCN4 current. The slope of the voltage dependence was somewhat steeper in hHCN2 (−6.3 ± 0.5 mV, n = 11) than in hHCN4 (−11.0 ± 0.6 mV, n = 20). It should be noted that the hHCN4 current did not reach a true steady state even after 3 s pulses. For this reason the actual difference in V1/2 and slope factor between hHCN2 and hHCN4 may be smaller than predicted from our fits. Both channels exhibited marked differences in their activation kinetics. The hHCN2 current (Figure 3A) activated much more quickly than the hHCN4 current (Figure 3B, note also the different time scale). The current traces of either channel type could be fitted with a single exponential function when the initial delay in current activation was excluded. Time constants for hHCN2 activation ranged from 196 ± 11 ms (n = 11) at −140 mV to 463 ± 45 ms (n = 11) at −110 mV. In contrast, hHCN4 activated with a distinctly slower time course with time constants ranging from 659 ± 49 ms (n = 16) at −140 mV to 23 ± 9 s at −110 mV (n = 16) (Figure 3D). Figure 3.Electrophysiological properties of the expressed hHCN2 and hHCN4 channel measured in whole-cell voltage clamp. (A) hHCN2 channel currents. Upper: voltage protocol. Cells were clamped from a holding potential of −40 mV to various voltages (−140 to −50 mV in 10 mV increments) for 1.5 s followed by a step to −140 mV. Lower: current traces of a cell expressing hHCN2. (B) hHCN4 channel currents. Upper: voltage protocol. As in (A) except for 3 s long prepulses. Lower: hHCN4 current traces. (C) Activation curves of hHCN2 (open circles) and hHCN4 (filled circles) currents. Tail currents measured immediately after the voltage step to −140 mV, arrow in (A) and (B), were normalized and plotted as a function of the preceding membrane potential. (D) Voltage dependence of activation kinetics. Current traces at voltages ranging from −140 to −110 mV from hHCN2- or hHCN4-expressing cells were fitted with a single exponential. The time constants for hHCN2 (open circles) and hHCN4 (filled circles) activation are plotted against the corresponding potentials. (E) Determination of the I/V relationship of the fully activated hHCN2 (open circle) and hHCN4 (filled circle) channel as described in the Materials and methods. Download figure Download PowerPoint To determine the ion selectivity of hHCN2 and hHCN4, we measured the current/voltage (I/V) relationship of the fully activated channels (Figure 3E). The reversal potential at 30 mM extracellular K+ was −23.8 ± 0.8 mV (n = 7) and −22.4 ± 2.2 mV (n = 6) for hHCN2 and hHCN4, respectively. The relative permeability ratio for Na+ versus K+ (PNa/PK), as determined by the Goldmann–Hodgkin–Katz equation, was 0.19 for hHCN2 and 0.22 for hHCN4, close to the value observed for native If. A well-established feature of the native pacemaker current is the enhancement by cAMP resulting from a shift of the activation curve towards positive voltages. In accordance with these findings, in whole-cell mode measurements cAMP increased both the hHCN2 and the hHCN4 current by shifting the activation curves 15–16 mV in the positive direction (Figure 4A and B). The V1/2 values for the hHCN2 and the hHCN4 current were −97 ± 1 mV (n = 11) and −109 ± 1 mV (n = 20) in the absence, and −81 ± 2 mV (n = 11) and −94 ± 3 mV (n = 16) in the presence of 1 mM cAMP, respectively. Analogous to the native If current (DiFrancesco, 1991), cAMP also accelerated the activation kinetics of both channel types (Figure 4C and D). The hHCN2 current induced by a step to −140 mV was fitted by a single exponential with a time constant of 179 ms in the absence and 69 ms in the presence of 1 mM intracellular cAMP. Similarly, the hHCN4 activation kinetics at −140 mV accelerated with a time constant of 679 ms in the absence to 447 ms in the presence of 1 mM cAMP. Cyclic AMP did not increase further the amplitude of either the hHCN2 or the hHCN4 current when the channels were fully activated by hyperpolarization. For example, the maximal current at −140 mV for hHCN4 was 1.5 ± 0.3 nA (n = 16) in the absence of cAMP and 1.2 ± 0.2 nA (n = 10) after perfusion with 1 mM cAMP. These values are not statistically different (p >0.05). Figure 4.Modulation of the hHCN2 and hHCN4 current by cAMP. (A, B) The voltage dependence of activation was determined from tail currents in whole-cell mode as described in legend to Figure 3C. (A) hHCN2 (open circles) and (B) hHCN4 currents (filled circles) were measured after intracellular perfusion for 1 min with 1 mM cAMP. For comparison the activation curves determined in the absence of cAMP (Figure 3C) are indicated by dotted lines. All curves represent fits of the tail currents by Boltzmann functions. (C, D) Acceleration of channel activation kinetics by cAMP. In whole-cell mode, the hHCN2 current (C) and the hHCN4 current (D) were fully activated by a step to −140 mV from a holding potential of −40 mV in the absence of cAMP (no symbol) or after intracellular perfusion for 1 min with 1 mM intracellular cAMP (open and filled circle, respectively). Normalized current traces are shown averaged from 11 cells under each condition (C) and from 16 and 10 cells in the absence respective presence of cAMP (D). (E, F) Direct modulation of hHCN2 (E) and hHCN4 (F) channels by cAMP measured in inside-out patches. Currents were evoked by clamping from a holding potential of −40 to −120 mV followed by a step (indicated by an arrow) to −150 mV. Traces were obtained in the absence (no symbol) and the presence of 10 μM cAMP in the bath solution [open circle (E), filled circle (F)]. Download figure Download PowerPoint Next, we determined the direct modulation of the two channels by cAMP using excised inside-out patches. In inside-out patches, V1/2 was shifted by ∼20–30 mV to more hyperpolarizing voltages with respect to the V1/2 measured in whole-cell mode. A similar phenomenon has been observed for expressed HCN channels (Ludwig et al., 1998) and also for the native If channel (DiFrancesco and Mangoni, 1994). Application of 10 μM cAMP to the intracellular side of the patch resulted in accelerated hHCN2 (Figure 4E) and hHCN4 (Figure 4F) channel opening at −120 and −150 mV. cAMP increased either current at −120 but not at −150 mV when the channels were already maximally activated by voltage. These results are in accordance with the effect of cAMP in whole-cell mode measurements (Figure 4A and B) showing a shift in the activation curve towards more positive voltages but no increase in the maximal current. Finally, we determined the blocking efficiency of Cs+ on the two pacemaker channels. The native If channel is blocked by low concentrations (0.1–5 mM) of extracellular Cs+ (DiFrancesco, 1982; Denyer and Brown, 1990). Similarly, the expressed hHCN2 and hHCN4 channels activated by a step to −140 mV were blocked between 80 and 90% by 0.5 mM Cs+ and ∼95% by 2 mM Cs+ (Figure 5A, B and E). In contrast, neither current was sensitive to 2 mM Ba2+ or 20 mM tetraethylammonium (TEA), two classic potassium channel blockers. Figure 5.(A, B) Effect of extracellular Cs+, TEA and Ba2+ on the two HCN currents. HHCN2 (A) and hHCN4 (B) currents were evoked from a holding potential of −40 mV with a step to −140 mV for 3 and 4 s, respectively. Current traces in the absence and presence of 0.5 and 2.0 mM extracellular Cs+ are shown. (C, D) Inhibition of whole-cell current by extracellular tetraethylammonium (TEA). hHCN2 (C) and hHCN4 (D) current traces in the absence and presence of 20 mM TEA are displayed. (E) Percentage inhibition of the current at −140 mV by 0.5 and 2 mM Cs+, 2 mM Ba2+ and 20 mM TEA. Inhibition of the hHCN2 and hHCN4 currents is represented by open and filled bars, respectively, with the number of experiments shown in brackets above. Download figure Download PowerPoint Discussion The molecular nature of the channel(s) involved in the cardiac If current was not known until now. Here, we report the cloning of two pacemaker channels from human heart, hHCN2 and hHCN4 with different activation kinetics. The hHCN2 clone has an overall sequence identity of 94% with mHCN2 from mouse brain (Ludwig et al., 1998). No tissue-dependent alternative splicing events have been detected in the large number of hHCN2 clones examined indicating that identical HCN2 channels are present in heart and brain. The hHCN4 channel probably represents the human full-length clone homologous to the partial cDNA sequence mBCNG-3 (Santoro et al., 1998). This partial cDNA isolated from mouse brain codes for 500 amino acids of the core region of a HCN channel and its sequence is 98% identical to hHCN4. The hHCN2 and hHCN4 cDNAs were cloned from a library prepared from human heart conduction tissue. The relative high expression of hHCN2 and hHCN4 mRNAs on Northern blots (Figure 2A and B) indicates that the two channels are expressed not only in the conduction tissues but also in contractile myocytes. This conclusion is consistent with the RT–PCR experiments demonstrating significant expression of both HCN mRNAs in cardiac ventricle and atrium. It is also compatible with previous results describing If currents in ventricular (Yu et al., 1995; Baker et al., 1997; Hoppe et al., 1998) and atrial (Thuringer et al., 1992; Porciatti et al., 1997) myocytes, as well as in the different conduction tissues (DiFrancesco, 1993). The ventricular myocyte If current has a V1/2 between −95 and −135 mV, which is consistent with the V1/2 values of the two HCN channels. In contrast, the If current of sinoatrial node cells displays a varying but distinctly more positive V1/2 ranging from −65 to −90 mV. The reason for the differing V1/2 values between If from ventricular and sinoatrial node myocytes is unknown. Both hHCN2 and hHCN4 exhibit properties corresponding to those of native If channels, i.e. activation at hyperpolarized membrane potentials, permeation of Na+ and K+, cAMP-dependent positive shift of the I/V relationship plus acceleration of channel activation and finally block by extracellular Cs+. However, apart from these basic characteristics the two channels display profoundly different activation kinetics. The hHCN2 channel activates comparably quickly, whereas hHCN4 activation is markedly slower. Two kinetically distinct components of native If with time constants in the range of hundreds of milliseconds and seconds, respectively, have been described in neurons (Solomon and Nerbonne, 1993) and heart cells (DiFrancesco et al., 1986; Maruoka et al., 1994; Liu et al., 1996). This raises the possibility that native If may be generated by the concerted action of two distinct channels. Moreover, the zebrafish mutant smo (Baker et al., 1997) displays a reduced heart rate due to a diminished If current. Smo cardiomyocytes show a severe reduction in the fast kinetic component of If (time constant ∼300 ms), whereas a slow component (time constant 2 s) remained unchanged. It was concluded that If is due to the activity of two channels and that the smo mutation selectively affects one of them. Taken together, the description of native If with two kinetic components and in particular the genetic evidence from the smo mutant strongly support the idea that two populations of channels with differing activation kinetics contribute to the If current. Hence, the results of this study suggest that HCN2 and HCN4 may underlie the fast respective slow component of If. The structural basis for the differing activation kinetics of the two channels is not yet known. The core region including the transmembrane segments, pore and CNBD is 90% homologous between the two channel types. An intriguing possibility is that the long N- or C-terminus of hHCN4 interacts with the channel activation mechanism slowing it down. Further experiments will be necessary to test this hypothesis. Native pacemaker channels probably form tetramers. The presence of both hHCN2 and hHCN4 mRNAs in cardiac tissues raises the possibility that the two channels may form heteromeric complexes in vivo. Although we can not rule out this possibility, our experimental findings do not support the existence of heteromeric channels. The expression of each subtype alone gave rise to functional channels indicating that the formation of hHCN2 and hHCN4 homomers is sufficient to make a pacemaker channel. Our preliminary analysis of currents induced by coexpression of hHCN2 and hHCN4 supports the notion that two distinct hHCN2 and hHCN4 channel populations are formed. In addition, the data from the smo mutant (Baker et al., 1997) favor the hypothesis that native pacemaker channels may be homomeric rather than heteromeric complexes. Several inherited disorders of pacemaking, such as various forms of congenital sinus node dysfunction have been described (Saracheck and Leonard, 1972; Lehmann and Klein, 1978; Mackintosh and Chamberlain, 1979). The determination of the complete exon–intron organization of hHCN2 should make it possible to screen genomic DNA samples of patients to detect possible links between these diseases and mutations of the hHCN2 gene. Naturally, the gene encoding hHCN4 will also be a candidate in the search for genes underlying defects of cardiac pacemaking. Gene targeting may help to elucidate the precise role and relative importance of the two HCN channels for normal pacemaking activity. Materials and methods Molecular cloning of hHCN2 and hHCN4 A cDNA library was prepared from the atrioventricular node region of a human heart using pcDNAII vector (Invitrogen). Colonies (1×106) were screened with a 32P-labeled probe corresponding to amino acids 96–566 of mHCN1 from mouse brain (Ludwig et al., 1998). Twenty-one independent clones were recovered and analyzed by restriction mapping and partial sequencing. Sixteen clones were classified as hHCN2, three of them contained the complete ORF. The length of the hHCN2 cDNA sequence was 3406 bp. Five clones fell into a separate class designated hHCN4, two of them contained the full-length coding sequence. The 3′-untranslated region (UTR) of HCN4 comprised ∼2.6 kb and was characterized by restriction mapping and partial sequencing. The total length of the hHCN4 cDNA was 6.8 kb. Two clones containing the entire ORF from each class were sequenced on both strands. Sequencing was carried out using a combination of manual sequencing with Sequenase v. 2.0 (Amersham) and automated sequencing on an ABI Prism 310 with BigDye terminator chemistry (Perkin Elmer) and a LI-COR 4200 system (Li-Cor Biotechnology). Genomic structure of hHCN2 The exon–intron organization of the human HCN2 gene was determined by comparing the hHCN2 cDNA with the sequence of cosmid clones F18382 (accession No. AC005559 for the telomeric and AC005577 for the centromeric part) and R33683 (accession No. AC004449) from the DDBJ/EMBL/GenBank database. The segment between cosmids F18382 and R33683 was PCR amplified with the primers HAC1F9 (5′-CGGCGCCAGTACCAGGAGAAGGT-3′) and HAC1R4 (5′-TCAGCATGGCCGTGACGAAGTTGG-3′). Cosmid R31514, which overlaps the above cosmid clones (http://www-bio.llnl.gov/bbrp/genome/genome.html) was used as template. The gap in the reported sequence of cosmid F18382 between the telomeric and centromeric" @default.
• W2171569887 created "2016-06-24" @default.
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• W2171569887 date "1999-05-04" @default.
• W2171569887 modified "2023-09-25" @default.
• W2171569887 title "Two pacemaker channels from human heart with profoundly different activation kinetics" @default.
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