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- W2171569896 abstract "A new method is described which permits staining of the histamine in mast cells of embedded tissue. Small specimens of fresh tissue, rapidly frozen in isopentane-liquid nitrogen, are dried in vacuo at –35°C, embedded in paraffin, sectioned and stained with o-phthalaldehyde in ethyl benzene. Mast cell histamine appears bright yellow as viewed under fluorescence microscopy. The procedure is standardized, suitable for a wide variety of tissues and remarkably free of diffusion artifacts. The uncut blocks may be stored for months with no changes in later histamine-staining potential. Alternatively, embedding may be done in polyethylene glycols with satisfactory results." @default.
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- W2171569896 date "1968-06-01" @default.
- W2171569896 modified "2023-10-16" @default.
- W2171569896 title "MAST CELL STAIN FOR HISTAMINE IN FREEZE-DRIED EMBEDDED TISSUE" @default.
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- W2171569896 doi "https://doi.org/10.1177/16.6.433" @default.
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