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- W2171685074 abstract "While soil enzymes have been explicitly included in the soil organic carbon (SOC) decomposition models, there is a serious lack of suitable data for model parameterization. This study provides well-documented enzymatic parameters for application in enzyme-driven SOC decomposition models from a compilation and analysis of published measurements. In particular, we developed appropriate kinetic parameters for five typical ligninolytic and cellulolytic enzymes (β-glucosidase, cellobiohydrolase, endo-glucanase, peroxidase, and phenol oxidase). The kinetic parameters included the maximum specific enzyme activity (Vmax) and half-saturation constant (Km) in the Michaelis–Menten equation. The activation energy (Ea) and the pH optimum and sensitivity (pHopt and pHsen) were also analyzed. pHsen was estimated by fitting an exponential-quadratic function. The Vmax values, often presented in different units under various measurement conditions, were converted into the same units at a reference temperature (20 °C) and pHopt. Major conclusions are: (i) Both Vmax and Km were log-normal distributed, with no significant difference in Vmax exhibited between enzymes originating from bacteria or fungi. (ii) No significant difference in Vmax was found between cellulases and ligninases; however, there was significant difference in Km between them. (iii) Ligninases had higher Ea values and lower pHopt than cellulases; average ratio of pHsen to pHopt ranged 0.3–0.4 for the five enzymes, which means that an increase or decrease of 1.1–1.7 pH units from pHopt would reduce Vmax by 50%. (iv) Our analysis indicated that the Vmax values from lab measurements with purified enzymes were 1–2 orders of magnitude higher than those for use in SOC decomposition models under field conditions." @default.
- W2171685074 created "2016-06-24" @default.
- W2171685074 creator A5008427918 @default.
- W2171685074 creator A5023041174 @default.
- W2171685074 creator A5027488510 @default.
- W2171685074 creator A5033036369 @default.
- W2171685074 creator A5062063360 @default.
- W2171685074 date "2012-05-01" @default.
- W2171685074 modified "2023-10-14" @default.
- W2171685074 title "Parameter estimation for models of ligninolytic and cellulolytic enzyme kinetics" @default.
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