FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171741806> ?p ?o ?g. }

• W2171741806 endingPage "604" @default.
• W2171741806 startingPage "595" @default.
• W2171741806 abstract "Thrombopoietin (TPO) is known to be involved in megakariocytopoesis, but its role in the control of ovarian function is unknown. The aims of this study were to determine whether TPO can regulate the proliferation, apoptosis and secretory activity of ovarian cells, to identify possible intracellular mediators of TPO action, especially protein kinase A (PKA), and to define their interrelationships within ovarian cells. We investigated the effect of TPO treatment (0, 1, 10 or 100 ng/ml) on the following characteristics of cultured porcine ovarian follicles, determined using SDS-PAGE and Western blotting, immunocytochemistry, RIA and ELISA: the expression of intracellular peptides associated with proliferation (PCNA), apoptosis (Bax), tyrosine kinase (TK, phosphotyrosine), Cdc2/p34 kinase, PKA and the transcription factor CREB-1, and the secretion of progesterone, androstenedione, estradiol-17β, oxytocin, inhibin A, inhibin B, IGF-I, transforming growth factor-2β (TGF-2β) and IGF-binding protein 3 (IGFBP-3). The involvement of PKA-dependent pathways was examined by evaluating the effect of a PKA blocker (KT5720, 1 μg/ml), either alone or in combination with TPO, on the parameters listed above. A TPO-induced increase in expression of PCNA, Bax, PKA, TK, Cdc2/p34 and CREB was observed. Furthermore, TPO was able to inhibit androstenedione, estradiol, TGF-2β and IGFBP-3 secretion, and to stimulate oxytocin, inhibin A, inhibin B and IGF-I secretion. Progesterone secretion was not stimulated. The PKA blocker KT5720, when given alone, reduced the expression of Bax and TGF-2β, augmented the expression of PKA, CREB and oxytocin, but did not influence the secretion of progesterone, androstenedione, estradiol, IGFBP-3, inhibins A and B or IGF-I. When given together with TPO, the PKA blocker prevented or reversed the action of TPO on PKA, CREB, androstenedione, estradiol, IGFBP-3, oxytocin, but not its effect on Bax, TGF-2β or inhibin B. On the other hand, treatment with KT5720 augmented the effect of TPO on progesterone, inhibin A and IGF-I. These results provide the first evidence that TPO may be a potent regulator of ovarian function (e.g. proliferation, apoptosis and the secretion of peptide hormones, steroids, growth factors and growth factor-binding protein, as well as of the expression of some intracellular messengers). Furthermore, they demonstrated the importance of PKA in controlling these functions and in mediating the effects of TPO on ovarian cells. It remains possible that other (TK- and Cdc2/p34-dependent) intracellular mechanisms are also involved in mediating TPO action on the ovary." @default.
• W2171741806 created "2016-06-24" @default.
• W2171741806 creator A5000793374 @default.
• W2171741806 creator A5009516974 @default.
• W2171741806 creator A5055232220 @default.
• W2171741806 creator A5060292243 @default.
• W2171741806 creator A5070499515 @default.
• W2171741806 creator A5070561327 @default.
• W2171741806 creator A5090562689 @default.
• W2171741806 date "2004-12-01" @default.
• W2171741806 modified "2023-10-10" @default.
• W2171741806 title "Thrombopoietin regulates proliferation, apoptosis, secretory activity and intracellular messengers in porcine ovarian follicular cells: involvement of protein kinase A" @default.
• W2171741806 cites W1531520176 @default.
• W2171741806 cites W1641964996 @default.
• W2171741806 cites W1966203663 @default.
• W2171741806 cites W1967901025 @default.
• W2171741806 cites W1973418526 @default.
• W2171741806 cites W1978313069 @default.
• W2171741806 cites W1985796758 @default.
• W2171741806 cites W1998965637 @default.
• W2171741806 cites W2008359656 @default.
• W2171741806 cites W2034428041 @default.
• W2171741806 cites W2055049591 @default.
• W2171741806 cites W2058575875 @default.
• W2171741806 cites W2074585597 @default.
• W2171741806 cites W2078175470 @default.
• W2171741806 cites W2080079821 @default.
• W2171741806 cites W2092156741 @default.
• W2171741806 cites W2094476084 @default.
• W2171741806 cites W2096053718 @default.
• W2171741806 cites W2099855228 @default.
• W2171741806 cites W2100837269 @default.
• W2171741806 cites W2112561535 @default.
• W2171741806 cites W2134047155 @default.
• W2171741806 cites W2144458701 @default.
• W2171741806 cites W2277356073 @default.
• W2171741806 cites W2405221536 @default.
• W2171741806 doi "https://doi.org/10.1677/joe.1.05763" @default.
• W2171741806 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15590985" @default.
• W2171741806 hasPublicationYear "2004" @default.
• W2171741806 type Work @default.
• W2171741806 sameAs 2171741806 @default.
• W2171741806 citedByCount "23" @default.
• W2171741806 countsByYear W21717418062012 @default.
• W2171741806 countsByYear W21717418062015 @default.
• W2171741806 countsByYear W21717418062016 @default.
• W2171741806 countsByYear W21717418062017 @default.
• W2171741806 countsByYear W21717418062018 @default.
• W2171741806 countsByYear W21717418062019 @default.
• W2171741806 countsByYear W21717418062022 @default.
• W2171741806 crossrefType "journal-article" @default.
• W2171741806 hasAuthorship W2171741806A5000793374 @default.
• W2171741806 hasAuthorship W2171741806A5009516974 @default.
• W2171741806 hasAuthorship W2171741806A5055232220 @default.
• W2171741806 hasAuthorship W2171741806A5060292243 @default.
• W2171741806 hasAuthorship W2171741806A5070499515 @default.
• W2171741806 hasAuthorship W2171741806A5070561327 @default.
• W2171741806 hasAuthorship W2171741806A5090562689 @default.
• W2171741806 hasBestOaLocation W21717418061 @default.
• W2171741806 hasConcept C104317684 @default.
• W2171741806 hasConcept C126322002 @default.
• W2171741806 hasConcept C134018914 @default.
• W2171741806 hasConcept C1629964 @default.
• W2171741806 hasConcept C184235292 @default.
• W2171741806 hasConcept C185592680 @default.
• W2171741806 hasConcept C55493867 @default.
• W2171741806 hasConcept C71924100 @default.
• W2171741806 hasConcept C86339819 @default.
• W2171741806 hasConcept C86803240 @default.
• W2171741806 hasConcept C95444343 @default.
• W2171741806 hasConcept C97029542 @default.
• W2171741806 hasConceptScore W2171741806C104317684 @default.
• W2171741806 hasConceptScore W2171741806C126322002 @default.
• W2171741806 hasConceptScore W2171741806C134018914 @default.
• W2171741806 hasConceptScore W2171741806C1629964 @default.
• W2171741806 hasConceptScore W2171741806C184235292 @default.
• W2171741806 hasConceptScore W2171741806C185592680 @default.
• W2171741806 hasConceptScore W2171741806C55493867 @default.
• W2171741806 hasConceptScore W2171741806C71924100 @default.
• W2171741806 hasConceptScore W2171741806C86339819 @default.
• W2171741806 hasConceptScore W2171741806C86803240 @default.
• W2171741806 hasConceptScore W2171741806C95444343 @default.
• W2171741806 hasConceptScore W2171741806C97029542 @default.
• W2171741806 hasIssue "3" @default.
• W2171741806 hasLocation W21717418061 @default.
• W2171741806 hasLocation W21717418062 @default.
• W2171741806 hasOpenAccess W2171741806 @default.
• W2171741806 hasPrimaryLocation W21717418061 @default.
• W2171741806 hasRelatedWork W1966504330 @default.
• W2171741806 hasRelatedWork W1966775726 @default.
• W2171741806 hasRelatedWork W1979139803 @default.
• W2171741806 hasRelatedWork W1991560548 @default.
• W2171741806 hasRelatedWork W2030889776 @default.
• W2171741806 hasRelatedWork W2037631372 @default.
• W2171741806 hasRelatedWork W2040058909 @default.
• W2171741806 hasRelatedWork W2063957970 @default.
• W2171741806 hasRelatedWork W2132898409 @default.
• W2171741806 hasRelatedWork W4249176446 @default.

• next