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• W2171795919 endingPage "38" @default.
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• W2171795919 abstract "The serum- and glucocorticoid-inducible kinase Sgk1 contributes to cardiac remodeling and development of heart failure, which is paralelled by Sgk1-dependent stimulation of the cardiac Na(+)/H(+) exchanger Nhe1. Glucocorticoids are powerful stimulators of Sgk1 expression and influence cardiac remodeling. The present study thus explored whether the glucocorticoid receptor agonist dexamethasone influenced cardiac Sgk1 expression, as well as activity, expression and phosphorylation at Ser(703) of the cardiac Na(+)/H(+) exchanger Nhe1.Experiments were performed in HL-1 cardiomyocytes and gene targeted mice lacking functional Sgk1 (sgk1(-/-)) and respective wild type mice (sgk1(+/+)). Gene expression was determined by quantitative RT-PCR and Nhe1 phosphorylation was determined utilizing a specific antibody against a 14-3-3 binding motif at P-Ser(703), which represents a putative phosphorylation site recognition motif for Sgk1 and is involved in Nhe1 activation. Cytosolic pH (pHi) was determined utilizing 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Nhe activity by the Na(+)-dependent realkalinization after an ammonium pulse.Treatment of HL-1 cardiomyocytes with dexamethasone was followed by a significant increase in Sgk1 mRNA expression, parallelled by increased Na(+)/H(+) exchanger activity. Furthermore, dexamethasone significantly increased Nhe1 and Spp1 mRNA expression. The effects of dexamethasone were blunted by cotreatment of HL-1 cardiomyocytes with the Sgk1 inhibitor EMD638683. Cotreatment with Nhe1 inhibitor cariporide similarly prevented dexamethasone-stimulated Spp1 mRNA expression. In sgk1(+/+) mice, dexamethasone significantly increased cardiac Sgk1 mRNA levels. In sgk1(+/+) mice, but not in sgk1(-/-) mice, dexamethasone significantly increased cardiac Nhe1 mRNA expression and Nhe1 phosphorylation at Ser(703). Furthermore, cardiac Spp1, Ctgf, Nppa and Nppb mRNA levels were significantly increased in dexamethasone treated sgk1(+/+) mice, effects significantly blunted in sgk1(-/-) mice.Sgk1 is critically involved in the phosphorylation and activation of the cardiac Na(+)/H(+) exchanger Nhe1." @default.
• W2171795919 created "2016-06-24" @default.
• W2171795919 creator A5004202808 @default.
• W2171795919 creator A5004630250 @default.
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• W2171795919 creator A5084208566 @default.
• W2171795919 creator A5090406770 @default.
• W2171795919 date "2013-01-01" @default.
• W2171795919 modified "2023-10-06" @default.
• W2171795919 title "Sgk1-Dependent Stimulation of Cardiac Na⁺/H⁺Exchanger Nhe1 by Dexamethasone" @default.
• W2171795919 cites W1525041374 @default.
• W2171795919 cites W1526510631 @default.
• W2171795919 cites W1558785883 @default.
• W2171795919 cites W1589657989 @default.
• W2171795919 cites W1603470245 @default.
• W2171795919 cites W1661647511 @default.
• W2171795919 cites W1841061580 @default.
• W2171795919 cites W1858389298 @default.
• W2171795919 cites W1859478751 @default.
• W2171795919 cites W1948948667 @default.
• W2171795919 cites W1965337411 @default.
• W2171795919 cites W1965980675 @default.
• W2171795919 cites W1967700835 @default.
• W2171795919 cites W1970718602 @default.
• W2171795919 cites W1972519966 @default.
• W2171795919 cites W1974194275 @default.
• W2171795919 cites W1976676963 @default.
• W2171795919 cites W1983311124 @default.
• W2171795919 cites W1984780819 @default.
• W2171795919 cites W1985562808 @default.
• W2171795919 cites W1988989702 @default.
• W2171795919 cites W1989282030 @default.
• W2171795919 cites W1989475487 @default.
• W2171795919 cites W1990862509 @default.
• W2171795919 cites W1993835711 @default.
• W2171795919 cites W1995365748 @default.
• W2171795919 cites W1995607268 @default.
• W2171795919 cites W1997013992 @default.
• W2171795919 cites W1997103341 @default.
• W2171795919 cites W1999413380 @default.
• W2171795919 cites W2001629228 @default.
• W2171795919 cites W2010346270 @default.
• W2171795919 cites W2010688793 @default.
• W2171795919 cites W2013425658 @default.
• W2171795919 cites W2017245231 @default.
• W2171795919 cites W2018114771 @default.
• W2171795919 cites W2023319730 @default.
• W2171795919 cites W2023470676 @default.
• W2171795919 cites W2023929054 @default.
• W2171795919 cites W2025058961 @default.
• W2171795919 cites W2025620168 @default.
• W2171795919 cites W2027030850 @default.
• W2171795919 cites W2028297894 @default.
• W2171795919 cites W2029032935 @default.
• W2171795919 cites W2031247901 @default.
• W2171795919 cites W2031609571 @default.
• W2171795919 cites W2033830300 @default.
• W2171795919 cites W2035168239 @default.
• W2171795919 cites W2040492681 @default.
• W2171795919 cites W2042943866 @default.
• W2171795919 cites W2048171645 @default.
• W2171795919 cites W2055335169 @default.
• W2171795919 cites W2057032201 @default.
• W2171795919 cites W2061560661 @default.
• W2171795919 cites W2065724905 @default.
• W2171795919 cites W2068527329 @default.
• W2171795919 cites W2072847837 @default.
• W2171795919 cites W2074752775 @default.
• W2171795919 cites W2074969314 @default.
• W2171795919 cites W2076825066 @default.
• W2171795919 cites W2077319362 @default.
• W2171795919 cites W2077626212 @default.
• W2171795919 cites W2079081079 @default.
• W2171795919 cites W2079519145 @default.
• W2171795919 cites W2079643837 @default.
• W2171795919 cites W2080557891 @default.
• W2171795919 cites W2084563588 @default.
• W2171795919 cites W2085749059 @default.
• W2171795919 cites W2086730539 @default.
• W2171795919 cites W2088689370 @default.
• W2171795919 cites W2088749722 @default.
• W2171795919 cites W2089162256 @default.
• W2171795919 cites W2089312027 @default.
• W2171795919 cites W2092412911 @default.
• W2171795919 cites W2092493326 @default.
• W2171795919 cites W2092512238 @default.
• W2171795919 cites W2093006711 @default.
• W2171795919 cites W2095427168 @default.
• W2171795919 cites W2095647887 @default.
• W2171795919 cites W2096939668 @default.
• W2171795919 cites W2098274706 @default.
• W2171795919 cites W2099672556 @default.
• W2171795919 cites W2101136596 @default.
• W2171795919 cites W2101744962 @default.

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