Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171810366> ?p ?o ?g. }
- W2171810366 endingPage "806" @default.
- W2171810366 startingPage "797" @default.
- W2171810366 abstract "Summary: A derivative of Bacillus megaterium QM B1551 cured of all seven resident plasmids was mutated to Lac–2. Transposon Tn917 carrying lacZ and cat was then used to isolate four spo: :lacZ-cat fusion mutants by screening for colonies expressing the fusion in stationary phase. The sporulation frequencies of the mutants ranged from 10–7 to 10–2. Macrolide, lincosamide and streptogramin B resistance (MLSr) of the mutants cotransduced 100% with the sporulation defect and all four mutations mapped near trp by transduction in the order: trp–hisH–spo. All four mutants isolated were defective early in sporulation since β-galactosidase could be detected between zero and 2 h after the end of exponential growth. Electron microscopy of two of the mutants expressing the enzyme at t1–t2 revealed a defect prior to or just at the beginning of septum formation in one, and after completion of septum formation in the other. Little or no synthesis of dipicolinic acid, glucose dehydrogenase or alkaline phosphatase was detected in the mutants, but each was neutral protease positive. These results show that the mutations were in at least two early genes expressed before glucose dehydrogenase production. This study represents the first genetic characterization of sporulation mutants in B. megaterium and also demonstrates that gene fusion technology can be used in this species." @default.
- W2171810366 created "2016-06-24" @default.
- W2171810366 creator A5009498403 @default.
- W2171810366 creator A5026414174 @default.
- W2171810366 date "1991-04-01" @default.
- W2171810366 modified "2023-09-26" @default.
- W2171810366 title "Isolation and characterization of sporulation lacZ fusion mutants of Bacillus megaterium" @default.
- W2171810366 cites W119765585 @default.
- W2171810366 cites W1480366252 @default.
- W2171810366 cites W1483853196 @default.
- W2171810366 cites W1486358076 @default.
- W2171810366 cites W1519737110 @default.
- W2171810366 cites W1521290780 @default.
- W2171810366 cites W1536539071 @default.
- W2171810366 cites W1546783991 @default.
- W2171810366 cites W1554313123 @default.
- W2171810366 cites W1563454467 @default.
- W2171810366 cites W156648096 @default.
- W2171810366 cites W1608457529 @default.
- W2171810366 cites W1618458920 @default.
- W2171810366 cites W1834062581 @default.
- W2171810366 cites W1900856863 @default.
- W2171810366 cites W1912305654 @default.
- W2171810366 cites W1921058700 @default.
- W2171810366 cites W1946218037 @default.
- W2171810366 cites W1964807917 @default.
- W2171810366 cites W1972981991 @default.
- W2171810366 cites W1986038245 @default.
- W2171810366 cites W1993042465 @default.
- W2171810366 cites W1996823807 @default.
- W2171810366 cites W2007432822 @default.
- W2171810366 cites W2008044598 @default.
- W2171810366 cites W2008570857 @default.
- W2171810366 cites W2014509207 @default.
- W2171810366 cites W2015764731 @default.
- W2171810366 cites W2024041553 @default.
- W2171810366 cites W2031227019 @default.
- W2171810366 cites W2061243460 @default.
- W2171810366 cites W2067184583 @default.
- W2171810366 cites W2070974154 @default.
- W2171810366 cites W2082748586 @default.
- W2171810366 cites W2106550529 @default.
- W2171810366 cites W2123812634 @default.
- W2171810366 cites W2140680463 @default.
- W2171810366 cites W2146953954 @default.
- W2171810366 cites W2275967469 @default.
- W2171810366 cites W2316397892 @default.
- W2171810366 cites W2334573075 @default.
- W2171810366 cites W2464846532 @default.
- W2171810366 cites W2483932544 @default.
- W2171810366 cites W2285660830 @default.
- W2171810366 cites W2301930450 @default.
- W2171810366 doi "https://doi.org/10.1099/00221287-137-4-797" @default.
- W2171810366 hasPublicationYear "1991" @default.
- W2171810366 type Work @default.
- W2171810366 sameAs 2171810366 @default.
- W2171810366 citedByCount "10" @default.
- W2171810366 countsByYear W21718103662014 @default.
- W2171810366 crossrefType "journal-article" @default.
- W2171810366 hasAuthorship W2171810366A5009498403 @default.
- W2171810366 hasAuthorship W2171810366A5026414174 @default.
- W2171810366 hasBestOaLocation W21718103661 @default.
- W2171810366 hasConcept C104317684 @default.
- W2171810366 hasConcept C143065580 @default.
- W2171810366 hasConcept C153911025 @default.
- W2171810366 hasConcept C170344550 @default.
- W2171810366 hasConcept C22744801 @default.
- W2171810366 hasConcept C2780989563 @default.
- W2171810366 hasConcept C523546767 @default.
- W2171810366 hasConcept C54355233 @default.
- W2171810366 hasConcept C55493867 @default.
- W2171810366 hasConcept C86803240 @default.
- W2171810366 hasConceptScore W2171810366C104317684 @default.
- W2171810366 hasConceptScore W2171810366C143065580 @default.
- W2171810366 hasConceptScore W2171810366C153911025 @default.
- W2171810366 hasConceptScore W2171810366C170344550 @default.
- W2171810366 hasConceptScore W2171810366C22744801 @default.
- W2171810366 hasConceptScore W2171810366C2780989563 @default.
- W2171810366 hasConceptScore W2171810366C523546767 @default.
- W2171810366 hasConceptScore W2171810366C54355233 @default.
- W2171810366 hasConceptScore W2171810366C55493867 @default.
- W2171810366 hasConceptScore W2171810366C86803240 @default.
- W2171810366 hasIssue "4" @default.
- W2171810366 hasLocation W21718103661 @default.
- W2171810366 hasOpenAccess W2171810366 @default.
- W2171810366 hasPrimaryLocation W21718103661 @default.
- W2171810366 hasRelatedWork W1571771606 @default.
- W2171810366 hasRelatedWork W1968976175 @default.
- W2171810366 hasRelatedWork W1992033009 @default.
- W2171810366 hasRelatedWork W2050897266 @default.
- W2171810366 hasRelatedWork W2111958127 @default.
- W2171810366 hasRelatedWork W2171329180 @default.
- W2171810366 hasRelatedWork W2414030161 @default.
- W2171810366 hasRelatedWork W2524850911 @default.
- W2171810366 hasRelatedWork W2620737981 @default.
- W2171810366 hasRelatedWork W3215086467 @default.
- W2171810366 hasVolume "137" @default.
- W2171810366 isParatext "false" @default.