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• W2171857319 abstract "<h3>Introduction</h3> <i>Helicobacter</i> species can initiate animal colitis similar to human ulcerative colitis (UC). <i>Campylobacter concisus</i> has been linked to paediatric Crohn9s disease (CD). Microaerophilic organisms such as <i>Helicobacter</i> and <i>Campylobacter</i> may be involved in the initiation of IBD. <h3>Methods</h3> Paediatric patients undergoing colonoscopy were recruited to two groups: those with a new diagnosis of IBD at their first presentation and controls with a macroscopically normal colon and no evidence of IBD on biopsy. All subjects were free from systemic antibiotics, steroids and immunosuppression for 3 months. 24 IBD patients and 26 controls were studied. The IBD cohort comprised 12 (50%) CD, 8 (33.3%) UC and 4 (16.7%) IBD unspecified (IBD-U) patients. 15 (62.5%) of the IBD and 20 (77.8%) of the control groups were male with median ages of 12.4 and 11.0 years, respectively. 5–6 Colonic mucosal biopsies were taken: in controls largely from the sigmoid/rectum and in IBD from the most distal inflamed site. 3 biopsies were stored at −80°C for subsequent DNA extraction and PCR. 1–2 biopsies were used for culture. 5 selective plates were used alongside blood agar. Cultures were incubated in microaerophilic conditions (Anoxomat) at 37°C. Plates were reviewed twice weekly for up to 1 month. Isolates deemed Gram-negative and microaerophilic underwent PCR of the 16S rRNA gene for phylogenetic identification. <h3>Results</h3> No <i>Helicobacter</i> were cultured. 3 <i>Campylobacter</i> spp. were isolated: <i>C concisus</i> from a subject with CD, <i>Campylobacter curvus</i> and <i>Campylobacter showae</i> from controls. <i>Sutterella wadsworthensis</i> was isolated from 13 subjects: 8 controls and 5 IBD. All biopsies were positive for bacterial DNA with universal primers. Nested PCR for <i>Helicobacter</i> genus was positive in 5 (10%) subjects, comprising 3 (12.5%) IBD and 2 (7.7%) controls. PCR for <i>Campylobacter</i> genus was positive in 38 (76%) subjects, comprising 19 (79.2%) IBD and 19 (73.1%) controls. Nested PCR for <i>C concisus</i> was positive in 25 (50%) subjects, comprising 14 (58.3%) IBD (8/12 CD, 3/8 UC and 3/4 IBD-U) and 11 (42.3%) controls. In response to frequent isolation of the organism, PCR for <i>S wadsworthensis</i> was positive in 48 (96%) subjects, comprising 23 (95.8%) IBD and 25 (96.2%) controls. <h3>Conclusion</h3> <i>Campylobacter</i> spp. and <i>Sutterella wadsworthensis</i> are commonly identified in the paediatric colon. <i>C concisus</i> appears more prevalent in CD although this was not significant. <i>Helicobacter</i> spp. are uncommon. PCR and culture methodology have revealed no significant distinction between the microaerophilic microbiota of paediatric IBD versus controls. It is unlikely that these organisms have a role in the initiation of paediatric IBD." @default.
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• W2171857319 date "2011-03-13" @default.
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• W2171857319 title "The role of microaerophilic colonic mucosal bacteria in de novo paediatric inflammatory bowel disease" @default.
• W2171857319 doi "https://doi.org/10.1136/gut.2011.239301.311" @default.
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