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- W2171882922 abstract "A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment ofLeishmania minicircle DNA was constructed. These primers amplified not only inter- but also intraspecifically polymorphic sequences. Individual sequences revealed a higher intraspecific than interspecific divergence. It is concluded that individual sequences are of limited relevance for species determination. In contrast, when a data base of 19 different sequences was analyzed in a dendrographic plot, an accurate species differentiation was feasible." @default.
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- W2171882922 date "1994-01-01" @default.
- W2171882922 modified "2023-10-16" @default.
- W2171882922 title "Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA" @default.
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- W2171882922 doi "https://doi.org/10.1007/bf00932693" @default.
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