Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171933896> ?p ?o ?g. }
- W2171933896 endingPage "5775" @default.
- W2171933896 startingPage "5764" @default.
- W2171933896 abstract "The eyes absent 1 protein (Eya1) plays an essential role in the development of various organs in both invertebrates and vertebrates. Mutations in the human EYA1 gene are linked to BOR (branchio-oto-renal) syndrome, characterized by kidney defects, hearing loss, and branchial arch anomalies. For a better understanding of Eya1's function, we have set out to identify new Eya1-interacting proteins. Here we report the identification of the related proteins Sipl1 (Shank-interacting protein-like 1) and Rbck1 (RBCC protein interacting with PKC1) as novel interaction partners of Eya1. We confirmed the interactions by glutathione S-transferase (GST) pulldown analysis and coimmunoprecipitation. A first mechanistic insight is provided by the demonstration that Sipl1 and Rbck1 enhance the function of Eya proteins to act as coactivators for the Six transcription factors. Using reverse transcriptase PCR (RT-PCR) and in situ hybridization, we show that Sipl1 and Rbck1 are coexpressed with Eya1 in several organs during embryogenesis of both the mouse and zebrafish. By morpholino-mediated knockdown, we demonstrate that the Sipl1 and Rbck1 orthologs are involved in different aspects of zebrafish development. In particular, knockdown of one Sipl1 ortholog as well as one Rbck1 ortholog led to a BOR syndrome-like phenotype, with characteristic defects in ear and branchial arch formation." @default.
- W2171933896 created "2016-06-24" @default.
- W2171933896 creator A5018408434 @default.
- W2171933896 creator A5028310893 @default.
- W2171933896 creator A5033312056 @default.
- W2171933896 creator A5045677007 @default.
- W2171933896 creator A5047313183 @default.
- W2171933896 creator A5063432504 @default.
- W2171933896 creator A5075279504 @default.
- W2171933896 creator A5080263701 @default.
- W2171933896 creator A5081030603 @default.
- W2171933896 date "2010-12-01" @default.
- W2171933896 modified "2023-10-16" @default.
- W2171933896 title "Sipl1 and Rbck1 Are Novel Eya1-Binding Proteins with a Role in Craniofacial Development" @default.
- W2171933896 cites W102944501 @default.
- W2171933896 cites W1529732178 @default.
- W2171933896 cites W1577373236 @default.
- W2171933896 cites W1769017161 @default.
- W2171933896 cites W1897589155 @default.
- W2171933896 cites W1976781053 @default.
- W2171933896 cites W1977624875 @default.
- W2171933896 cites W1987590454 @default.
- W2171933896 cites W1992086025 @default.
- W2171933896 cites W1993074652 @default.
- W2171933896 cites W1996223704 @default.
- W2171933896 cites W1996243898 @default.
- W2171933896 cites W1997245179 @default.
- W2171933896 cites W1997339096 @default.
- W2171933896 cites W1998851985 @default.
- W2171933896 cites W2001175271 @default.
- W2171933896 cites W2001199164 @default.
- W2171933896 cites W2003996311 @default.
- W2171933896 cites W2008737180 @default.
- W2171933896 cites W2010837729 @default.
- W2171933896 cites W2013599007 @default.
- W2171933896 cites W2016194944 @default.
- W2171933896 cites W2021052287 @default.
- W2171933896 cites W2023581846 @default.
- W2171933896 cites W2025047543 @default.
- W2171933896 cites W2030617924 @default.
- W2171933896 cites W2034397996 @default.
- W2171933896 cites W2050270368 @default.
- W2171933896 cites W2055515723 @default.
- W2171933896 cites W2062749633 @default.
- W2171933896 cites W2070156564 @default.
- W2171933896 cites W2073632680 @default.
- W2171933896 cites W2074036892 @default.
- W2171933896 cites W2078785528 @default.
- W2171933896 cites W2079485641 @default.
- W2171933896 cites W2083125769 @default.
- W2171933896 cites W2085206941 @default.
- W2171933896 cites W2089459899 @default.
- W2171933896 cites W2090707442 @default.
- W2171933896 cites W2095056735 @default.
- W2171933896 cites W2095803633 @default.
- W2171933896 cites W2107277218 @default.
- W2171933896 cites W2108053853 @default.
- W2171933896 cites W2120147422 @default.
- W2171933896 cites W2121278418 @default.
- W2171933896 cites W2122329737 @default.
- W2171933896 cites W2122506888 @default.
- W2171933896 cites W2125349610 @default.
- W2171933896 cites W2128374908 @default.
- W2171933896 cites W2129477962 @default.
- W2171933896 cites W2134371698 @default.
- W2171933896 cites W2141858057 @default.
- W2171933896 cites W2148059499 @default.
- W2171933896 cites W2148886011 @default.
- W2171933896 cites W2150683921 @default.
- W2171933896 cites W2152032402 @default.
- W2171933896 cites W2152509610 @default.
- W2171933896 cites W2153833981 @default.
- W2171933896 cites W2156361642 @default.
- W2171933896 cites W2157439965 @default.
- W2171933896 cites W2158304082 @default.
- W2171933896 doi "https://doi.org/10.1128/mcb.01645-09" @default.
- W2171933896 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3004267" @default.
- W2171933896 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20956555" @default.
- W2171933896 hasPublicationYear "2010" @default.
- W2171933896 type Work @default.
- W2171933896 sameAs 2171933896 @default.
- W2171933896 citedByCount "43" @default.
- W2171933896 countsByYear W21719338962012 @default.
- W2171933896 countsByYear W21719338962013 @default.
- W2171933896 countsByYear W21719338962014 @default.
- W2171933896 countsByYear W21719338962015 @default.
- W2171933896 countsByYear W21719338962016 @default.
- W2171933896 countsByYear W21719338962017 @default.
- W2171933896 countsByYear W21719338962018 @default.
- W2171933896 countsByYear W21719338962019 @default.
- W2171933896 countsByYear W21719338962020 @default.
- W2171933896 countsByYear W21719338962021 @default.
- W2171933896 countsByYear W21719338962022 @default.
- W2171933896 countsByYear W21719338962023 @default.
- W2171933896 crossrefType "journal-article" @default.
- W2171933896 hasAuthorship W2171933896A5018408434 @default.
- W2171933896 hasAuthorship W2171933896A5028310893 @default.
- W2171933896 hasAuthorship W2171933896A5033312056 @default.