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- W2172026557 abstract "An in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented." @default.
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- W2172026557 date "2005-01-01" @default.
- W2172026557 modified "2023-09-27" @default.
- W2172026557 title "Anti-HIV-1 activities in extracts from some medicinal plants as assessed in anin vitro biochemical HIV-1 reverse transcriptase assay" @default.
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- W2172026557 doi "https://doi.org/10.1002/ptr.1536" @default.
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