Matches in SemOpenAlex for { <https://semopenalex.org/work/W2172037013> ?p ?o ?g. }
- W2172037013 endingPage "750" @default.
- W2172037013 startingPage "737" @default.
- W2172037013 abstract "BTEX compounds such as benzene are frequent soil and groundwater contaminants that are easily biodegraded under oxic conditions by bacteria. In contrast, benzene is rather recalcitrant under anaerobic conditions. The analysis of anoxic degradation is often hampered by difficult sampling conditions, limited amounts of biomass and interference of matrix compounds with proteomic approaches. In order to improve the procedure for protein extraction we established a scheme consisting of the following steps: dissociation of cells from lava granules, cell lysis by ultrasonication and purification of proteins by phenol extraction. The 2D-gels revealed a resolution of about 240 proteins spots and the spot patterns showed strong matrix dependence, but still differences were detectable between the metaproteomes obtained after growth on benzene and benzoate. Using direct data base search as well as de novo sequencing approaches we were able to identify several proteins. An enoyl-CoA hydratase with cross species homology to Azoarcus evansii, is known to be involved in the anoxic degradation of xenobiotics. Thereby the identification confirmed that this procedure has the capacity to analyse the metaproteome of an anoxic living microbial community." @default.
- W2172037013 created "2016-06-24" @default.
- W2172037013 creator A5000266633 @default.
- W2172037013 creator A5005911295 @default.
- W2172037013 creator A5009760942 @default.
- W2172037013 creator A5018412626 @default.
- W2172037013 creator A5049644125 @default.
- W2172037013 creator A5060849662 @default.
- W2172037013 creator A5068106192 @default.
- W2172037013 creator A5075153084 @default.
- W2172037013 creator A5086113901 @default.
- W2172037013 date "2009-04-21" @default.
- W2172037013 modified "2023-10-17" @default.
- W2172037013 title "Improving protein extraction and separation methods for investigating the metaproteome of anaerobic benzene communities within sediments" @default.
- W2172037013 cites W1507573241 @default.
- W2172037013 cites W1518992342 @default.
- W2172037013 cites W1520694472 @default.
- W2172037013 cites W1554701319 @default.
- W2172037013 cites W1666163025 @default.
- W2172037013 cites W170515146 @default.
- W2172037013 cites W1928916941 @default.
- W2172037013 cites W1964313477 @default.
- W2172037013 cites W1965263082 @default.
- W2172037013 cites W1967511506 @default.
- W2172037013 cites W1970172743 @default.
- W2172037013 cites W1970488850 @default.
- W2172037013 cites W1974865973 @default.
- W2172037013 cites W1978877803 @default.
- W2172037013 cites W1981384832 @default.
- W2172037013 cites W1985246182 @default.
- W2172037013 cites W1987583427 @default.
- W2172037013 cites W1988581694 @default.
- W2172037013 cites W1992783987 @default.
- W2172037013 cites W1993959886 @default.
- W2172037013 cites W2009460670 @default.
- W2172037013 cites W2012155062 @default.
- W2172037013 cites W2018223047 @default.
- W2172037013 cites W2027055846 @default.
- W2172037013 cites W2029330897 @default.
- W2172037013 cites W2030005001 @default.
- W2172037013 cites W2038615001 @default.
- W2172037013 cites W2042365454 @default.
- W2172037013 cites W2042438516 @default.
- W2172037013 cites W2044853699 @default.
- W2172037013 cites W2048333869 @default.
- W2172037013 cites W2051340819 @default.
- W2172037013 cites W2052854891 @default.
- W2172037013 cites W2054645658 @default.
- W2172037013 cites W2066361645 @default.
- W2172037013 cites W2066672290 @default.
- W2172037013 cites W2070711445 @default.
- W2172037013 cites W2081563254 @default.
- W2172037013 cites W2086540936 @default.
- W2172037013 cites W2089740378 @default.
- W2172037013 cites W2098003280 @default.
- W2172037013 cites W2100837269 @default.
- W2172037013 cites W2115704904 @default.
- W2172037013 cites W2122401417 @default.
- W2172037013 cites W2123260358 @default.
- W2172037013 cites W2135343770 @default.
- W2172037013 cites W2136981365 @default.
- W2172037013 cites W2139574832 @default.
- W2172037013 cites W2145656870 @default.
- W2172037013 cites W2149339305 @default.
- W2172037013 cites W2150166775 @default.
- W2172037013 cites W2151041036 @default.
- W2172037013 cites W2151139697 @default.
- W2172037013 cites W2155252735 @default.
- W2172037013 cites W2161093517 @default.
- W2172037013 cites W4214911971 @default.
- W2172037013 cites W4236797742 @default.
- W2172037013 cites W4293247451 @default.
- W2172037013 doi "https://doi.org/10.1007/s10532-009-9261-3" @default.
- W2172037013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2847156" @default.
- W2172037013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19381451" @default.
- W2172037013 hasPublicationYear "2009" @default.
- W2172037013 type Work @default.
- W2172037013 sameAs 2172037013 @default.
- W2172037013 citedByCount "70" @default.
- W2172037013 countsByYear W21720370132012 @default.
- W2172037013 countsByYear W21720370132013 @default.
- W2172037013 countsByYear W21720370132014 @default.
- W2172037013 countsByYear W21720370132015 @default.
- W2172037013 countsByYear W21720370132016 @default.
- W2172037013 countsByYear W21720370132017 @default.
- W2172037013 countsByYear W21720370132018 @default.
- W2172037013 countsByYear W21720370132019 @default.
- W2172037013 countsByYear W21720370132020 @default.
- W2172037013 countsByYear W21720370132021 @default.
- W2172037013 countsByYear W21720370132022 @default.
- W2172037013 crossrefType "journal-article" @default.
- W2172037013 hasAuthorship W2172037013A5000266633 @default.
- W2172037013 hasAuthorship W2172037013A5005911295 @default.
- W2172037013 hasAuthorship W2172037013A5009760942 @default.
- W2172037013 hasAuthorship W2172037013A5018412626 @default.
- W2172037013 hasAuthorship W2172037013A5049644125 @default.
- W2172037013 hasAuthorship W2172037013A5060849662 @default.
- W2172037013 hasAuthorship W2172037013A5068106192 @default.