FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2172108144> ?p ?o ?g. }

• W2172108144 endingPage "811" @default.
• W2172108144 startingPage "803" @default.
• W2172108144 abstract "Mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1) underlie some familial cases of amyotrophic lateral sclerosis (ALS), a neurodegenerative disorder characterized by loss of cortical, brainstem and spinal motoneurons. Transgenic mice over- expressing a mutated form of human SOD1 containing a Gly-->Ala substitution at position 93 (SOD1(G93A)) develop a severe, progressive motoneuron disease. We investigated the potential of recombinant adeno-associated virus (rAAV) to transfer neuroprotective molecules in this animal ALS model. Initial experiments showed that injection of an rAAV vector encoding green fluorescent protein unilaterally into the lumbar spinal cord of wild-type mice leads to expression of the reporter gene in 34.7 +/- 5.2% of the motoneurons surrounding the injection site. Intraspinal injection of an rAAV encoding the anti-apoptotic protein bcl-2 in SOD1 (G93A) mice resulted in sustained bcl-2 expression in motoneurons and significantly increased the number of surviving motoneurons at the end-stage of disease. Moreover, the compound muscle action potential amplitude elicited by nerve stimulation and recorded by electromyographic measurements was higher in the rAAV-bcl-2-treated group than in controls. Local bcl-2 expression in spinal motoneurons delayed the appearance of signs of motor deficiency but was not sufficient to prolong the survival of SOD1 (G93A) mice. To our know-ledge, this study describes the first successful transduction and protection of spinal motoneurons by direct gene transfer in a model of progressive motoneuron disease. Our results support the use of AAVs for the delivery of protective genes to spinal cord moto-neurons as a possible way to enhance motoneuron survival and repair." @default.
• W2172108144 created "2016-06-24" @default.
• W2172108144 creator A5001089917 @default.
• W2172108144 creator A5001356149 @default.
• W2172108144 creator A5009387400 @default.
• W2172108144 creator A5014922008 @default.
• W2172108144 creator A5029251301 @default.
• W2172108144 creator A5041127003 @default.
• W2172108144 date "2000-03-22" @default.
• W2172108144 modified "2023-09-26" @default.
• W2172108144 title "Increased motoneuron survival and improved neuromuscular function in transgenic ALS mice after intraspinal injection of an adeno-associated virus encoding Bcl-2" @default.
• W2172108144 cites W1530779137 @default.
• W2172108144 cites W1532194993 @default.
• W2172108144 cites W1541751114 @default.
• W2172108144 cites W1545372895 @default.
• W2172108144 cites W1555605403 @default.
• W2172108144 cites W1565043637 @default.
• W2172108144 cites W1598808289 @default.
• W2172108144 cites W1942870285 @default.
• W2172108144 cites W1971832103 @default.
• W2172108144 cites W1974697456 @default.
• W2172108144 cites W1978559408 @default.
• W2172108144 cites W1994396884 @default.
• W2172108144 cites W1995686474 @default.
• W2172108144 cites W1997170151 @default.
• W2172108144 cites W1997208169 @default.
• W2172108144 cites W1997577743 @default.
• W2172108144 cites W1999261266 @default.
• W2172108144 cites W2001436105 @default.
• W2172108144 cites W2019820968 @default.
• W2172108144 cites W2022367303 @default.
• W2172108144 cites W2035133362 @default.
• W2172108144 cites W2035504758 @default.
• W2172108144 cites W2037473273 @default.
• W2172108144 cites W2038036971 @default.
• W2172108144 cites W2041838043 @default.
• W2172108144 cites W2042598793 @default.
• W2172108144 cites W2049983049 @default.
• W2172108144 cites W2053040219 @default.
• W2172108144 cites W2056326326 @default.
• W2172108144 cites W2058334706 @default.
• W2172108144 cites W2059195318 @default.
• W2172108144 cites W2061131610 @default.
• W2172108144 cites W2061219878 @default.
• W2172108144 cites W2062728160 @default.
• W2172108144 cites W2063772445 @default.
• W2172108144 cites W2068201081 @default.
• W2172108144 cites W2070243481 @default.
• W2172108144 cites W2073898426 @default.
• W2172108144 cites W2074531504 @default.
• W2172108144 cites W2076359142 @default.
• W2172108144 cites W2084419598 @default.
• W2172108144 cites W2090099409 @default.
• W2172108144 cites W2096386031 @default.
• W2172108144 cites W2101056451 @default.
• W2172108144 cites W2107768418 @default.
• W2172108144 cites W2121944814 @default.
• W2172108144 cites W2123413118 @default.
• W2172108144 cites W2125501179 @default.
• W2172108144 cites W2126608948 @default.
• W2172108144 cites W2172154292 @default.
• W2172108144 cites W2396470512 @default.
• W2172108144 cites W2414458688 @default.
• W2172108144 cites W70578846 @default.
• W2172108144 doi "https://doi.org/10.1093/hmg/9.5.803" @default.
• W2172108144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10749988" @default.
• W2172108144 hasPublicationYear "2000" @default.
• W2172108144 type Work @default.
• W2172108144 sameAs 2172108144 @default.
• W2172108144 citedByCount "127" @default.
• W2172108144 countsByYear W21721081442012 @default.
• W2172108144 countsByYear W21721081442013 @default.
• W2172108144 countsByYear W21721081442014 @default.
• W2172108144 countsByYear W21721081442015 @default.
• W2172108144 countsByYear W21721081442016 @default.
• W2172108144 countsByYear W21721081442017 @default.
• W2172108144 countsByYear W21721081442018 @default.
• W2172108144 countsByYear W21721081442019 @default.
• W2172108144 countsByYear W21721081442020 @default.
• W2172108144 countsByYear W21721081442021 @default.
• W2172108144 countsByYear W21721081442022 @default.
• W2172108144 countsByYear W21721081442023 @default.
• W2172108144 crossrefType "journal-article" @default.
• W2172108144 hasAuthorship W2172108144A5001089917 @default.
• W2172108144 hasAuthorship W2172108144A5001356149 @default.
• W2172108144 hasAuthorship W2172108144A5009387400 @default.
• W2172108144 hasAuthorship W2172108144A5014922008 @default.
• W2172108144 hasAuthorship W2172108144A5029251301 @default.
• W2172108144 hasAuthorship W2172108144A5041127003 @default.
• W2172108144 hasBestOaLocation W21721081442 @default.
• W2172108144 hasConcept C102230213 @default.
• W2172108144 hasConcept C104317684 @default.
• W2172108144 hasConcept C111599444 @default.
• W2172108144 hasConcept C142724271 @default.
• W2172108144 hasConcept C169760540 @default.
• W2172108144 hasConcept C25498285 @default.
• W2172108144 hasConcept C2776752467 @default.
• W2172108144 hasConcept C2777615887 @default.

• next