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- W2172170716 abstract "Insulin stimulates translocation of GLUT4 storage vesicles (GSVs) to the surface of adipocytes, but precisely where insulin acts is controversial. Here we quantify the size, dynamics, and frequency of single vesicle exocytosis in 3T3-L1 adipocytes. We use a new GSV reporter, VAMP2-pHluorin, and bypass insulin signaling by disrupting the GLUT4-retention protein TUG. Remarkably, in unstimulated TUG-depleted cells, the exocytic rate is similar to that in insulin-stimulated control cells. In TUG-depleted cells, insulin triggers a transient, twofold burst of exocytosis. Surprisingly, insulin promotes fusion pore expansion, blocked by acute perturbation of phospholipase D, which reflects both properties intrinsic to the mobilized vesicles and a novel regulatory site at the fusion pore itself. Prolonged stimulation causes cargo to switch from ∼60 nm GSVs to larger exocytic vesicles characteristic of endosomes. Our results support a model whereby insulin promotes exocytic flux primarily by releasing an intracellular brake, but also by accelerating plasma membrane fusion and switching vesicle traffic between two distinct circuits." @default.
- W2172170716 created "2016-06-24" @default.
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- W2172170716 date "2011-05-09" @default.
- W2172170716 modified "2023-10-01" @default.
- W2172170716 title "Dual-mode of insulin action controls GLUT4 vesicle exocytosis" @default.
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- W2172170716 doi "https://doi.org/10.1083/jcb.201008135" @default.
- W2172170716 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3166865" @default.
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