FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2172201964> ?p ?o ?g. }

• W2172201964 abstract "Abstract Background The highly conserved macromolecular synthesis operon (MMSO) contains both dnaG (primase) and sigA (primary sigma factor). However, in previously evaluated gram-positive species, the MMSO is divergent upstream of dnaG . The MMSO of Bacillus subtilis contains three open reading frames (ORFs) that are differentially regulated by multiple promoters. In conjunction with studies to determine the expression profile of dnaG , the MMSO of Staphylococus epidermidis was characterized. Results The ORFs of S. epidermidis were compared to the previously described MMSO of B. subtilis and two additional ORFs in S. epidermidis, serp1129 and serp1130 , were identified. The largest transcript, 4.8 kb in length, was expressed only in exponential growth and encompassed all four ORFs ( serp1130, serp1129, dnaG , and sigA ). A separate transcript (1.5 kb) comprising serp1130 and serp1129 was expressed in early exponential growth. Two smaller transcripts 1.3 and 1.2 kb in size were detected with a sigA probe in both exponential and post-exponential phases of growth. Western blot analysis correlated with the transcriptional profile and demonstrated that Serp1129 was detected only in the exponential phase of growth. Computational analysis identified that Serp1130 contained a CBS motif whereas Serp1129 contained an ATP/GTP binding motif. Functional studies of Serp1129 demonstrated that it was capable of binding both ATP and GTP. Comparisons with a sigB:dhfr mutant revealed that the 1.3 kb sigA transcript was regulated by a σ B -dependent promoter. Conclusions These studies demonstrated that the S. epidermidis 1457 MMSO contains two ORFs ( serp1129 and serp1130 ) not described within the B. subtilis MMSO and at least three promoters, one of which is σ β -dependent. The transcriptional regulation of sigA by σ B provides evidence that the staphylococcal σ B -dependent response is controlled at both the transcriptional and post-transcriptional level. The conservation of serp1129 across multiple gram-positive organisms and its capability to bind ATP and GTP support the need for further investigation of its role in bacterial growth." @default.
• W2172201964 created "2016-06-24" @default.
• W2172201964 creator A5007609770 @default.
• W2172201964 creator A5020947009 @default.
• W2172201964 creator A5023867416 @default.
• W2172201964 creator A5079465120 @default.
• W2172201964 creator A5085800731 @default.
• W2172201964 date "2010-01-12" @default.
• W2172201964 modified "2023-09-25" @default.
• W2172201964 title "Genetic analysis of the Staphylococcus epidermidis Macromolecular Synthesis Operon: Serp1129 is an ATP binding protein and sigA transcription is regulated by both σA- and σB-dependent promoters" @default.
• W2172201964 cites W1528583344 @default.
• W2172201964 cites W1536724064 @default.
• W2172201964 cites W1595558935 @default.
• W2172201964 cites W1858717470 @default.
• W2172201964 cites W1982366830 @default.
• W2172201964 cites W1990721890 @default.
• W2172201964 cites W1991075336 @default.
• W2172201964 cites W2002044386 @default.
• W2172201964 cites W2011696195 @default.
• W2172201964 cites W2019073608 @default.
• W2172201964 cites W2020468241 @default.
• W2172201964 cites W2021054184 @default.
• W2172201964 cites W2044618828 @default.
• W2172201964 cites W2046304613 @default.
• W2172201964 cites W2047009593 @default.
• W2172201964 cites W2051563423 @default.
• W2172201964 cites W2055209115 @default.
• W2172201964 cites W2069267306 @default.
• W2172201964 cites W2080007096 @default.
• W2172201964 cites W2082424083 @default.
• W2172201964 cites W2095685144 @default.
• W2172201964 cites W2097493383 @default.
• W2172201964 cites W2106727737 @default.
• W2172201964 cites W2110755993 @default.
• W2172201964 cites W2112822852 @default.
• W2172201964 cites W2115366678 @default.
• W2172201964 cites W2118273311 @default.
• W2172201964 cites W2120160920 @default.
• W2172201964 cites W2126485894 @default.
• W2172201964 cites W2140187633 @default.
• W2172201964 cites W2147057508 @default.
• W2172201964 cites W2148253865 @default.
• W2172201964 cites W2154901929 @default.
• W2172201964 cites W2154978372 @default.
• W2172201964 cites W2162846954 @default.
• W2172201964 cites W2166714586 @default.
• W2172201964 cites W2171046836 @default.
• W2172201964 cites W4240372082 @default.
• W2172201964 doi "https://doi.org/10.1186/1471-2180-10-8" @default.
• W2172201964 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2824700" @default.
• W2172201964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20067631" @default.
• W2172201964 hasPublicationYear "2010" @default.
• W2172201964 type Work @default.
• W2172201964 sameAs 2172201964 @default.
• W2172201964 citedByCount "1" @default.
• W2172201964 countsByYear W21722019642014 @default.
• W2172201964 crossrefType "journal-article" @default.
• W2172201964 hasAuthorship W2172201964A5007609770 @default.
• W2172201964 hasAuthorship W2172201964A5020947009 @default.
• W2172201964 hasAuthorship W2172201964A5023867416 @default.
• W2172201964 hasAuthorship W2172201964A5079465120 @default.
• W2172201964 hasAuthorship W2172201964A5085800731 @default.
• W2172201964 hasBestOaLocation W21722019641 @default.
• W2172201964 hasConcept C101762097 @default.
• W2172201964 hasConcept C104317684 @default.
• W2172201964 hasConcept C138885662 @default.
• W2172201964 hasConcept C143065580 @default.
• W2172201964 hasConcept C150194340 @default.
• W2172201964 hasConcept C153911025 @default.
• W2172201964 hasConcept C167625842 @default.
• W2172201964 hasConcept C179926584 @default.
• W2172201964 hasConcept C188192080 @default.
• W2172201964 hasConcept C203075996 @default.
• W2172201964 hasConcept C2777949923 @default.
• W2172201964 hasConcept C2779489039 @default.
• W2172201964 hasConcept C2780530800 @default.
• W2172201964 hasConcept C41895202 @default.
• W2172201964 hasConcept C47289529 @default.
• W2172201964 hasConcept C47319516 @default.
• W2172201964 hasConcept C523546767 @default.
• W2172201964 hasConcept C54355233 @default.
• W2172201964 hasConcept C58167604 @default.
• W2172201964 hasConcept C73573662 @default.
• W2172201964 hasConcept C86803240 @default.
• W2172201964 hasConceptScore W2172201964C101762097 @default.
• W2172201964 hasConceptScore W2172201964C104317684 @default.
• W2172201964 hasConceptScore W2172201964C138885662 @default.
• W2172201964 hasConceptScore W2172201964C143065580 @default.
• W2172201964 hasConceptScore W2172201964C150194340 @default.
• W2172201964 hasConceptScore W2172201964C153911025 @default.
• W2172201964 hasConceptScore W2172201964C167625842 @default.
• W2172201964 hasConceptScore W2172201964C179926584 @default.
• W2172201964 hasConceptScore W2172201964C188192080 @default.
• W2172201964 hasConceptScore W2172201964C203075996 @default.
• W2172201964 hasConceptScore W2172201964C2777949923 @default.
• W2172201964 hasConceptScore W2172201964C2779489039 @default.
• W2172201964 hasConceptScore W2172201964C2780530800 @default.
• W2172201964 hasConceptScore W2172201964C41895202 @default.
• W2172201964 hasConceptScore W2172201964C47289529 @default.
• W2172201964 hasConceptScore W2172201964C47319516 @default.

• next