FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2172280799> ?p ?o ?g. }

• W2172280799 endingPage "1951" @default.
• W2172280799 startingPage "1943" @default.
• W2172280799 abstract "Cytochrome P450 (CYP) is one of the most important drug-metabolizing enzyme families, which participates in the biotransformation of many endogenous and exogenous compounds. Quantitative analysis of CYP expression levels is important when studying the efficacy of new drug molecules and assessing drug–drug interactions in drug development. At present, chemical probe-based assay is the most widely used approach for the evaluation of CYP activity although there are cross-reactions between the isoforms with high sequence homologies. Therefore, quantification of each isozyme is highly desired in regard to meeting the ever-increasing requirements for carrying out pharmacokinetics and personalized medicine in the academic, pharmaceutical, and clinical setting. Herein, an absolute quantification method was employed for the analysis of the seven isoforms CYP1A2, 2B6, 3A4, 3A5, 2C9, 2C19, and 2E1 using a proteome-derived approach in combination with stable isotope dilution assay. The average absolute amount measured from twelve human liver microsomes samples were 39.3, 4.3, 54.0, 4.6, 10.3, 3.0, and 9.3 (pmol/mg protein) for 1A2, 2B6, 3A4, 3A5, 2C9, 2C19, and 2E1, respectively. Importantly, the expression level of CYP3A4 showed high correlation (r = 0.943, p < 0.0001) with the functional activity, which was measured using bufalin—a highly selective chemical probe we have developed. The combination of MRM identification and analysis of the functional activity, as in the case of CYP3A4, provides a protocol which can be extended to other functional enzyme studies with wide application in pharmaceutical research." @default.
• W2172280799 created "2016-06-24" @default.
• W2172280799 creator A5004775159 @default.
• W2172280799 creator A5005171833 @default.
• W2172280799 creator A5030756098 @default.
• W2172280799 creator A5038556283 @default.
• W2172280799 creator A5054069282 @default.
• W2172280799 creator A5057839636 @default.
• W2172280799 creator A5070018729 @default.
• W2172280799 creator A5084095542 @default.
• W2172280799 creator A5085353674 @default.
• W2172280799 date "2014-07-14" @default.
• W2172280799 modified "2023-10-11" @default.
• W2172280799 title "Quantitative analysis of cytochrome P450 isoforms in human liver microsomes by the combination of proteomics and chemical probe-based assay" @default.
• W2172280799 cites W1545913859 @default.
• W2172280799 cites W1924733673 @default.
• W2172280799 cites W1963601997 @default.
• W2172280799 cites W1963797800 @default.
• W2172280799 cites W1964552738 @default.
• W2172280799 cites W1967791337 @default.
• W2172280799 cites W1973277206 @default.
• W2172280799 cites W1981563176 @default.
• W2172280799 cites W1981740820 @default.
• W2172280799 cites W1983060510 @default.
• W2172280799 cites W1986908417 @default.
• W2172280799 cites W1988463445 @default.
• W2172280799 cites W1989538460 @default.
• W2172280799 cites W1989654080 @default.
• W2172280799 cites W2007159204 @default.
• W2172280799 cites W2022839498 @default.
• W2172280799 cites W2032060308 @default.
• W2172280799 cites W2035465414 @default.
• W2172280799 cites W2041640706 @default.
• W2172280799 cites W2049033995 @default.
• W2172280799 cites W2053378327 @default.
• W2172280799 cites W2061779560 @default.
• W2172280799 cites W2066199197 @default.
• W2172280799 cites W2075259948 @default.
• W2172280799 cites W2075702840 @default.
• W2172280799 cites W2086502487 @default.
• W2172280799 cites W2095955511 @default.
• W2172280799 cites W2099832389 @default.
• W2172280799 cites W2100742272 @default.
• W2172280799 cites W2106281143 @default.
• W2172280799 cites W2119143636 @default.
• W2172280799 cites W2125940454 @default.
• W2172280799 cites W2127296393 @default.
• W2172280799 cites W2138332753 @default.
• W2172280799 cites W2139808687 @default.
• W2172280799 cites W2150957222 @default.
• W2172280799 cites W2157049173 @default.
• W2172280799 cites W2163536623 @default.
• W2172280799 cites W2164705071 @default.
• W2172280799 cites W3104902391 @default.
• W2172280799 cites W4245911931 @default.
• W2172280799 doi "https://doi.org/10.1002/pmic.201400025" @default.
• W2172280799 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24920405" @default.
• W2172280799 hasPublicationYear "2014" @default.
• W2172280799 type Work @default.
• W2172280799 sameAs 2172280799 @default.
• W2172280799 citedByCount "28" @default.
• W2172280799 countsByYear W21722807992014 @default.
• W2172280799 countsByYear W21722807992015 @default.
• W2172280799 countsByYear W21722807992016 @default.
• W2172280799 countsByYear W21722807992017 @default.
• W2172280799 countsByYear W21722807992018 @default.
• W2172280799 countsByYear W21722807992019 @default.
• W2172280799 countsByYear W21722807992020 @default.
• W2172280799 countsByYear W21722807992021 @default.
• W2172280799 countsByYear W21722807992023 @default.
• W2172280799 crossrefType "journal-article" @default.
• W2172280799 hasAuthorship W2172280799A5004775159 @default.
• W2172280799 hasAuthorship W2172280799A5005171833 @default.
• W2172280799 hasAuthorship W2172280799A5030756098 @default.
• W2172280799 hasAuthorship W2172280799A5038556283 @default.
• W2172280799 hasAuthorship W2172280799A5054069282 @default.
• W2172280799 hasAuthorship W2172280799A5057839636 @default.
• W2172280799 hasAuthorship W2172280799A5070018729 @default.
• W2172280799 hasAuthorship W2172280799A5084095542 @default.
• W2172280799 hasAuthorship W2172280799A5085353674 @default.
• W2172280799 hasConcept C104317684 @default.
• W2172280799 hasConcept C109650736 @default.
• W2172280799 hasConcept C119795356 @default.
• W2172280799 hasConcept C181199279 @default.
• W2172280799 hasConcept C185592680 @default.
• W2172280799 hasConcept C2780035454 @default.
• W2172280799 hasConcept C526171541 @default.
• W2172280799 hasConcept C53345823 @default.
• W2172280799 hasConcept C55493867 @default.
• W2172280799 hasConcept C70721500 @default.
• W2172280799 hasConcept C86745063 @default.
• W2172280799 hasConcept C86803240 @default.
• W2172280799 hasConcept C87644729 @default.
• W2172280799 hasConcept C89311334 @default.
• W2172280799 hasConcept C98274493 @default.
• W2172280799 hasConceptScore W2172280799C104317684 @default.
• W2172280799 hasConceptScore W2172280799C109650736 @default.
• W2172280799 hasConceptScore W2172280799C119795356 @default.

• next