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• W2172922925 abstract "The muscarinic receptor-mediated activation of the potassium current IK(ACh) in atrial myocytes is the most extensively studied prototype of the direct activation of effectors by G-proteins (reviewed in Szabo and Otero 1990; Birnbaumer et al. 1990). Electrophysiological methods applied both at the level of whole cell and single channel recording have shown that this is a membrane-delimited process that operates without diffusible cytosolic intermediates (Soejima and Noma 1984). The high sensitivity and time resolution inherent in the patch clamp technique (Hamill et al. 1981) make it possible to follow the time course of activation and deactivation of the effectors, in this case a specific class of K+-permeable channels, in response to rapid application and removal of agonist. Coupled with maneuvers that specifically alter receptor or G-protein function, these methods can be used to examine in a quantitative manner the kinetics of the processes interposed between receptor activation and channel opening.KeywordsPertussis ToxinFast KineticAtrial CellAtrial MyocytesMuscarinic AgonistThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
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• W2172922925 date "1993-01-01" @default.
• W2172922925 modified "2023-09-27" @default.
• W2172922925 title "Fast Kinetics of G-Protein Function In Vivo" @default.
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• W2172922925 doi "https://doi.org/10.1007/978-3-642-78345-6_18" @default.
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