FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2173021795> ?p ?o ?g. }

• W2173021795 endingPage "59" @default.
• W2173021795 startingPage "53" @default.
• W2173021795 abstract "Serological assays for the diagnosis of toxoplasmosis mostly rely on the tachyzoite specific antigens of Toxoplasma gondii, which are difficult to produce by conventional methods. The aim of this study was to clone and express of GRA7 protein of T. gondii and evaluate its potential for immunodiagnosis of toxoplasmosis in cancer patients. As well as validate the results using a new molecular assay, LAMP technique. The GRA7 gene was successfully cloned, expressed and purified by affinity chromatography and the production was evaluated by SDS PAGE, dot blot and western blot analyses. The rGRA7 was used for developing an ELISA based on the rGRA7 using sera from patients with toxoplasmosis and healthy controls. Furthermore, 50 serum samples from leukemic children infected with toxoplasmosis and 50 seronegative controls were included to evaluate the sensitivity and specificity of rGRA7 based ELISA. Finally, the LAMP technique was used to assess the accuracy and validity of the results obtained by rGRA7 based ELISA. The consistency of the results of two tests was determined by using the Kappa coefficient of agreement. The rGRA7 showed higher and optimum immunoreactivity with 1:100 dilution of serum from Toxoplasma infected patients. The sensitivity and specificity of test were calculated as 92 and 94%, respectively. According to the Kappa coefficient of agreement, there was a significant conformance between the results obtained by ELISA based on the rGRA7 and the results of LAMP technique (≈96%, P<0.001). Findings of the present study showed that rGRA7 can be used as a potential immunogenic antigen for developing immunodiagnostic tools for immunodiagnosis of toxoplasmosis in patients including patients with cancer." @default.
• W2173021795 created "2016-06-24" @default.
• W2173021795 creator A5000917810 @default.
• W2173021795 creator A5018915389 @default.
• W2173021795 creator A5035547947 @default.
• W2173021795 creator A5036075891 @default.
• W2173021795 creator A5058355922 @default.
• W2173021795 date "2016-02-01" @default.
• W2173021795 modified "2023-10-07" @default.
• W2173021795 title "Immunodiagnosis and molecular validation of Toxoplasma gondii-recombinant dense granular (GRA) 7 protein for the detection of toxoplasmosis in patients with cancer" @default.
• W2173021795 cites W1580003617 @default.
• W2173021795 cites W1591678521 @default.
• W2173021795 cites W1968417770 @default.
• W2173021795 cites W1974002584 @default.
• W2173021795 cites W1975801865 @default.
• W2173021795 cites W1979974453 @default.
• W2173021795 cites W1980779814 @default.
• W2173021795 cites W1985554305 @default.
• W2173021795 cites W1985615831 @default.
• W2173021795 cites W1992453374 @default.
• W2173021795 cites W1994831575 @default.
• W2173021795 cites W1994912334 @default.
• W2173021795 cites W2001207812 @default.
• W2173021795 cites W2021470461 @default.
• W2173021795 cites W2026999939 @default.
• W2173021795 cites W2048677372 @default.
• W2173021795 cites W2067335416 @default.
• W2173021795 cites W2068352389 @default.
• W2173021795 cites W2071709045 @default.
• W2173021795 cites W2071940916 @default.
• W2173021795 cites W2073416195 @default.
• W2173021795 cites W2105275554 @default.
• W2173021795 cites W2118948605 @default.
• W2173021795 cites W2149402888 @default.
• W2173021795 cites W2150243696 @default.
• W2173021795 cites W2166802410 @default.
• W2173021795 cites W2167658681 @default.
• W2173021795 cites W2168379579 @default.
• W2173021795 cites W2314062314 @default.
• W2173021795 cites W2749767897 @default.
• W2173021795 cites W292315508 @default.
• W2173021795 cites W4249788278 @default.
• W2173021795 doi "https://doi.org/10.1016/j.micres.2015.11.006" @default.
• W2173021795 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26805618" @default.
• W2173021795 hasPublicationYear "2016" @default.
• W2173021795 type Work @default.
• W2173021795 sameAs 2173021795 @default.
• W2173021795 citedByCount "31" @default.
• W2173021795 countsByYear W21730217952016 @default.
• W2173021795 countsByYear W21730217952017 @default.
• W2173021795 countsByYear W21730217952018 @default.
• W2173021795 countsByYear W21730217952019 @default.
• W2173021795 countsByYear W21730217952020 @default.
• W2173021795 countsByYear W21730217952021 @default.
• W2173021795 countsByYear W21730217952022 @default.
• W2173021795 countsByYear W21730217952023 @default.
• W2173021795 crossrefType "journal-article" @default.
• W2173021795 hasAuthorship W2173021795A5000917810 @default.
• W2173021795 hasAuthorship W2173021795A5018915389 @default.
• W2173021795 hasAuthorship W2173021795A5035547947 @default.
• W2173021795 hasAuthorship W2173021795A5036075891 @default.
• W2173021795 hasAuthorship W2173021795A5058355922 @default.
• W2173021795 hasBestOaLocation W21730217951 @default.
• W2173021795 hasConcept C104317684 @default.
• W2173021795 hasConcept C147483822 @default.
• W2173021795 hasConcept C153911025 @default.
• W2173021795 hasConcept C159047783 @default.
• W2173021795 hasConcept C159654299 @default.
• W2173021795 hasConcept C203014093 @default.
• W2173021795 hasConcept C2776415932 @default.
• W2173021795 hasConcept C2778062946 @default.
• W2173021795 hasConcept C2778128430 @default.
• W2173021795 hasConcept C40767141 @default.
• W2173021795 hasConcept C45189115 @default.
• W2173021795 hasConcept C54355233 @default.
• W2173021795 hasConcept C86803240 @default.
• W2173021795 hasConceptScore W2173021795C104317684 @default.
• W2173021795 hasConceptScore W2173021795C147483822 @default.
• W2173021795 hasConceptScore W2173021795C153911025 @default.
• W2173021795 hasConceptScore W2173021795C159047783 @default.
• W2173021795 hasConceptScore W2173021795C159654299 @default.
• W2173021795 hasConceptScore W2173021795C203014093 @default.
• W2173021795 hasConceptScore W2173021795C2776415932 @default.
• W2173021795 hasConceptScore W2173021795C2778062946 @default.
• W2173021795 hasConceptScore W2173021795C2778128430 @default.
• W2173021795 hasConceptScore W2173021795C40767141 @default.
• W2173021795 hasConceptScore W2173021795C45189115 @default.
• W2173021795 hasConceptScore W2173021795C54355233 @default.
• W2173021795 hasConceptScore W2173021795C86803240 @default.
• W2173021795 hasLocation W21730217951 @default.
• W2173021795 hasLocation W21730217952 @default.
• W2173021795 hasOpenAccess W2173021795 @default.
• W2173021795 hasPrimaryLocation W21730217951 @default.
• W2173021795 hasRelatedWork W1975098398 @default.
• W2173021795 hasRelatedWork W2170137230 @default.
• W2173021795 hasRelatedWork W2334959363 @default.
• W2173021795 hasRelatedWork W2380129376 @default.
• W2173021795 hasRelatedWork W2909651959 @default.

• next