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- W2173846013 abstract "While cilia are recognized as important signaling organelles, the extent of ciliary functions remains unknown because of difficulties in cataloguing proteins from mammalian primary cilia. We present a method that readily captures rapid snapshots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted proximity labeling enzyme (cilia-APEX). Besides identifying known ciliary proteins, cilia-APEX uncovered several ciliary signaling molecules. The kinases PKA, AMPK, and LKB1 were validated as bona fide ciliary proteins and PKA was found to regulate Hedgehog signaling in primary cilia. Furthermore, proteomics profiling of Ift27/Bbs19 mutant cilia correctly detected BBSome accumulation inside Ift27(-/-) cilia and revealed that β-arrestin 2 and the viral receptor CAR are candidate cargoes of the BBSome. This work demonstrates that proximity labeling can be applied to proteomics of non-membrane-enclosed organelles and suggests that proteomics profiling of cilia will enable a rapid and powerful characterization of ciliopathies." @default.
- W2173846013 created "2016-06-24" @default.
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- W2173846013 date "2015-11-01" @default.
- W2173846013 modified "2023-10-10" @default.
- W2173846013 title "Proteomics of Primary Cilia by Proximity Labeling" @default.
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- W2173846013 doi "https://doi.org/10.1016/j.devcel.2015.10.015" @default.
- W2173846013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4662609" @default.
- W2173846013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26585297" @default.
- W2173846013 hasPublicationYear "2015" @default.
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