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- W2174078204 abstract "The variable domains of antibodies can be expressed as a fusion with pIII, a minor coat protein of the bateriophage M13, for the generation of phage-display antibody reagents. The phage-display system is routinely used to enrich for recombinant antibodies against a specific target antigen from highly diverse naïve and immune libraries. Often once binders are selected, they are expressed as soluble proteins; however, it can be advantageous to use the phage-displayed antibody fragment as a reagent in binding assays. The repeating subunits of the viral capsid allows for significant signal amplification of binding events in downstream assays when utilizing a reporter-conjugated secondary antibody specific for the M13 capsid. Alternatively, labeling of the viral capsid with dyes or biotin molecules provides additional methods of achieving signal amplification in a variety of assay formats. The following protocols detail the use of phage-displayed single domain antibodies in sandwich assays for antigen detection." @default.
- W2174078204 created "2016-06-24" @default.
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- W2174078204 date "2013-10-30" @default.
- W2174078204 modified "2023-09-25" @default.
- W2174078204 title "Phage-Displayed Single Domain Antibodies as Recognition Elements" @default.
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- W2174078204 doi "https://doi.org/10.1007/978-1-62703-751-8_15" @default.
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