FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2174121805> ?p ?o ?g. }

• W2174121805 endingPage "421" @default.
• W2174121805 startingPage "411" @default.
• W2174121805 abstract "Mammalian 2-cysteine peroxiredoxins (Prxs) are susceptible to hyperoxidation by excess H2O2. The cytoplasmic family member Prx2 hyperoxidizes more readily than mitochondrial Prx3 due to slower dimerization of the sulfenic acid (SpOH) intermediate. Four variant amino acids near the C-terminus have been shown to contribute to this difference. We have performed kinetic analysis of the relationship between hyperoxidation and disulfide formation, using whole-protein MS and comparing wild-type (WT) Prx2 and Prx3 with tail-swap mutants in which the four amino acids were reversed. These changes make Prx3 more sensitive and Prx2 less sensitive to hyperoxidation and accounted for ∼70% of the difference between the two proteins. The tail swap mutant of Prx3 was also more susceptible when expressed in the mitochondria of HeLa cells. The hyperoxidized product at lower excesses of H2O2 was a semi-hyperoxidized dimer with one active site disulfide and the other a sulfinic acid. For Prx2, increasing the H2O2 concentration resulted in complete hyperoxidation. In contrast, only approximately half the Prx3 active sites underwent hyperoxidation and, even with high H2O2, the predominant product was the hyperoxidized dimer. Size exclusion chromatography (SEC) showed that the oligomeric forms of all redox states of Prx3 dissociated more readily into dimeric units than their Prx2 counterparts. Notably the species with one disulfide and one hyperoxidized active site was decameric for Prx2 and dimeric for Prx3. Reduction and re-oxidation of the hyperoxidized dimer of Prx3 produced hyperoxidized monomers, implying dissociation and rearrangement of the subunits of the functional homodimer." @default.
• W2174121805 created "2016-06-24" @default.
• W2174121805 creator A5012115253 @default.
• W2174121805 creator A5017354834 @default.
• W2174121805 creator A5017959770 @default.
• W2174121805 creator A5043961236 @default.
• W2174121805 creator A5050294423 @default.
• W2174121805 creator A5090966947 @default.
• W2174121805 date "2016-02-09" @default.
• W2174121805 modified "2023-10-15" @default.
• W2174121805 title "Kinetic analysis of structural influences on the susceptibility of peroxiredoxins 2 and 3 to hyperoxidation" @default.
• W2174121805 cites W122110278 @default.
• W2174121805 cites W1872295493 @default.
• W2174121805 cites W1974658879 @default.
• W2174121805 cites W1975984844 @default.
• W2174121805 cites W1976006396 @default.
• W2174121805 cites W1977793339 @default.
• W2174121805 cites W1981112109 @default.
• W2174121805 cites W1984387620 @default.
• W2174121805 cites W1985397824 @default.
• W2174121805 cites W1987686004 @default.
• W2174121805 cites W1991482163 @default.
• W2174121805 cites W1992669214 @default.
• W2174121805 cites W2005840706 @default.
• W2174121805 cites W2007684558 @default.
• W2174121805 cites W2016031481 @default.
• W2174121805 cites W2019123322 @default.
• W2174121805 cites W2022309155 @default.
• W2174121805 cites W2022504555 @default.
• W2174121805 cites W2027756065 @default.
• W2174121805 cites W2032612468 @default.
• W2174121805 cites W2040163846 @default.
• W2174121805 cites W2043346183 @default.
• W2174121805 cites W2043929237 @default.
• W2174121805 cites W2044075759 @default.
• W2174121805 cites W2050956358 @default.
• W2174121805 cites W2051687003 @default.
• W2174121805 cites W2062901635 @default.
• W2174121805 cites W2065427954 @default.
• W2174121805 cites W2069282308 @default.
• W2174121805 cites W2069480316 @default.
• W2174121805 cites W2082318697 @default.
• W2174121805 cites W2088411222 @default.
• W2174121805 cites W2088564722 @default.
• W2174121805 cites W2089818796 @default.
• W2174121805 cites W2095145021 @default.
• W2174121805 cites W2098048169 @default.
• W2174121805 cites W2130942310 @default.
• W2174121805 cites W2144157852 @default.
• W2174121805 cites W2146604272 @default.
• W2174121805 cites W2147228426 @default.
• W2174121805 cites W2152557671 @default.
• W2174121805 cites W2153831810 @default.
• W2174121805 cites W2167752118 @default.
• W2174121805 cites W2313280651 @default.
• W2174121805 cites W412918832 @default.
• W2174121805 cites W834967220 @default.
• W2174121805 doi "https://doi.org/10.1042/bj20150572" @default.
• W2174121805 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4859152" @default.
• W2174121805 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26614766" @default.
• W2174121805 hasPublicationYear "2016" @default.
• W2174121805 type Work @default.
• W2174121805 sameAs 2174121805 @default.
• W2174121805 citedByCount "28" @default.
• W2174121805 countsByYear W21741218052016 @default.
• W2174121805 countsByYear W21741218052017 @default.
• W2174121805 countsByYear W21741218052018 @default.
• W2174121805 countsByYear W21741218052019 @default.
• W2174121805 countsByYear W21741218052020 @default.
• W2174121805 countsByYear W21741218052021 @default.
• W2174121805 countsByYear W21741218052022 @default.
• W2174121805 countsByYear W21741218052023 @default.
• W2174121805 crossrefType "journal-article" @default.
• W2174121805 hasAuthorship W2174121805A5012115253 @default.
• W2174121805 hasAuthorship W2174121805A5017354834 @default.
• W2174121805 hasAuthorship W2174121805A5017959770 @default.
• W2174121805 hasAuthorship W2174121805A5043961236 @default.
• W2174121805 hasAuthorship W2174121805A5050294423 @default.
• W2174121805 hasAuthorship W2174121805A5090966947 @default.
• W2174121805 hasBestOaLocation W21741218051 @default.
• W2174121805 hasConcept C12554922 @default.
• W2174121805 hasConcept C162008176 @default.
• W2174121805 hasConcept C178790620 @default.
• W2174121805 hasConcept C181199279 @default.
• W2174121805 hasConcept C185592680 @default.
• W2174121805 hasConcept C2779201268 @default.
• W2174121805 hasConcept C2779546866 @default.
• W2174121805 hasConcept C2780158455 @default.
• W2174121805 hasConcept C2780416994 @default.
• W2174121805 hasConcept C28859421 @default.
• W2174121805 hasConcept C41183919 @default.
• W2174121805 hasConcept C515207424 @default.
• W2174121805 hasConcept C55493867 @default.
• W2174121805 hasConcept C86803240 @default.
• W2174121805 hasConceptScore W2174121805C12554922 @default.
• W2174121805 hasConceptScore W2174121805C162008176 @default.
• W2174121805 hasConceptScore W2174121805C178790620 @default.
• W2174121805 hasConceptScore W2174121805C181199279 @default.

• next