FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2174256332> ?p ?o ?g. }

• W2174256332 abstract "Inter-cellular communication is an integral part of a complex system that helps in maintaining basic cellular activities. As a result, the malfunctioning of such signaling can lead to many disorders. To understand cell-to-cell signaling, it is essential to study the spatial and temporal nature of the secreted molecules from the cell without disturbing the local environment. Various assays have been developed to study protein secretion, however, these methods are typically based on fluorescent probes which disrupt the relevant signaling pathways. To overcome this limitation, a label-free technique is required. In this paper, we describe the fabrication and application of a label-free localized surface plasmon resonance imaging (LSPRi) technology capable of detecting protein secretions from a single cell. The plasmonic nanostructures are lithographically patterned onto a standard glass coverslip and can be excited using visible light on commercially available light microscopes. Only a small fraction of the coverslip is covered by the nanostructures and hence this technique is well suited for combining common techniques such as fluorescence and bright-field imaging. A multidisciplinary approach is used in this protocol which incorporates sensor nanofabrication and subsequent biofunctionalization, binding kinetics characterization of ligand and analyte, the integration of the chip and live cells, and the analysis of the measured signal. As a whole, this technology enables a general label-free approach towards mapping cellular secretions and correlating them with the responses of nearby cells." @default.
• W2174256332 created "2016-06-24" @default.
• W2174256332 creator A5024525825 @default.
• W2174256332 creator A5042140497 @default.
• W2174256332 creator A5045573940 @default.
• W2174256332 creator A5071371676 @default.
• W2174256332 creator A5091203971 @default.
• W2174256332 date "2015-11-23" @default.
• W2174256332 modified "2023-09-25" @default.
• W2174256332 title "A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions" @default.
• W2174256332 cites W1593314378 @default.
• W2174256332 cites W1931315964 @default.
• W2174256332 cites W1966646357 @default.
• W2174256332 cites W1971868340 @default.
• W2174256332 cites W1983183460 @default.
• W2174256332 cites W1983970047 @default.
• W2174256332 cites W1989353204 @default.
• W2174256332 cites W1989559294 @default.
• W2174256332 cites W1999244169 @default.
• W2174256332 cites W2006882472 @default.
• W2174256332 cites W2010879932 @default.
• W2174256332 cites W2025712131 @default.
• W2174256332 cites W2043486114 @default.
• W2174256332 cites W2051051956 @default.
• W2174256332 cites W2063518906 @default.
• W2174256332 cites W2069190985 @default.
• W2174256332 cites W2088497878 @default.
• W2174256332 cites W2088857778 @default.
• W2174256332 cites W2093816239 @default.
• W2174256332 cites W2117370635 @default.
• W2174256332 cites W2129379081 @default.
• W2174256332 cites W2139174650 @default.
• W2174256332 cites W2145013849 @default.
• W2174256332 cites W2159188461 @default.
• W2174256332 cites W2159193579 @default.
• W2174256332 cites W2165032821 @default.
• W2174256332 cites W2166180044 @default.
• W2174256332 cites W2322237012 @default.
• W2174256332 cites W2324521257 @default.
• W2174256332 doi "https://doi.org/10.3791/53120" @default.
• W2174256332 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4692743" @default.
• W2174256332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26650542" @default.
• W2174256332 hasPublicationYear "2015" @default.
• W2174256332 type Work @default.
• W2174256332 sameAs 2174256332 @default.
• W2174256332 citedByCount "3" @default.
• W2174256332 countsByYear W21742563322017 @default.
• W2174256332 countsByYear W21742563322020 @default.
• W2174256332 crossrefType "journal-article" @default.
• W2174256332 hasAuthorship W2174256332A5024525825 @default.
• W2174256332 hasAuthorship W2174256332A5042140497 @default.
• W2174256332 hasAuthorship W2174256332A5045573940 @default.
• W2174256332 hasAuthorship W2174256332A5071371676 @default.
• W2174256332 hasAuthorship W2174256332A5091203971 @default.
• W2174256332 hasBestOaLocation W21742563321 @default.
• W2174256332 hasConcept C10390740 @default.
• W2174256332 hasConcept C106847996 @default.
• W2174256332 hasConcept C110879396 @default.
• W2174256332 hasConcept C120665830 @default.
• W2174256332 hasConcept C121332964 @default.
• W2174256332 hasConcept C12554922 @default.
• W2174256332 hasConcept C147080431 @default.
• W2174256332 hasConcept C147789679 @default.
• W2174256332 hasConcept C1491633281 @default.
• W2174256332 hasConcept C15526883 @default.
• W2174256332 hasConcept C155672457 @default.
• W2174256332 hasConcept C171250308 @default.
• W2174256332 hasConcept C185592680 @default.
• W2174256332 hasConcept C186060115 @default.
• W2174256332 hasConcept C192562407 @default.
• W2174256332 hasConcept C2776950831 @default.
• W2174256332 hasConcept C41008148 @default.
• W2174256332 hasConcept C49040817 @default.
• W2174256332 hasConcept C55493867 @default.
• W2174256332 hasConcept C86803240 @default.
• W2174256332 hasConceptScore W2174256332C10390740 @default.
• W2174256332 hasConceptScore W2174256332C106847996 @default.
• W2174256332 hasConceptScore W2174256332C110879396 @default.
• W2174256332 hasConceptScore W2174256332C120665830 @default.
• W2174256332 hasConceptScore W2174256332C121332964 @default.
• W2174256332 hasConceptScore W2174256332C12554922 @default.
• W2174256332 hasConceptScore W2174256332C147080431 @default.
• W2174256332 hasConceptScore W2174256332C147789679 @default.
• W2174256332 hasConceptScore W2174256332C1491633281 @default.
• W2174256332 hasConceptScore W2174256332C15526883 @default.
• W2174256332 hasConceptScore W2174256332C155672457 @default.
• W2174256332 hasConceptScore W2174256332C171250308 @default.
• W2174256332 hasConceptScore W2174256332C185592680 @default.
• W2174256332 hasConceptScore W2174256332C186060115 @default.
• W2174256332 hasConceptScore W2174256332C192562407 @default.
• W2174256332 hasConceptScore W2174256332C2776950831 @default.
• W2174256332 hasConceptScore W2174256332C41008148 @default.
• W2174256332 hasConceptScore W2174256332C49040817 @default.
• W2174256332 hasConceptScore W2174256332C55493867 @default.
• W2174256332 hasConceptScore W2174256332C86803240 @default.
• W2174256332 hasIssue "105" @default.
• W2174256332 hasLocation W21742563321 @default.
• W2174256332 hasLocation W21742563322 @default.
• W2174256332 hasLocation W21742563323 @default.
• W2174256332 hasLocation W21742563324 @default.

• next