FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2174396521> ?p ?o ?g. }

• W2174396521 endingPage "602" @default.
• W2174396521 startingPage "589" @default.
• W2174396521 abstract "Reduced or absent cytotrophoblast invasion of the maternal uterine spiral arterioles is a common clinical finding in studies of pregnancies complicated by preeclampsia, suggesting that the mechanisms mediating invasion of these cells is perturbed. The placenta initially develops in a low oxygen environment of 1–2% oxygen until after the 10th week of pregnancy. During this time oxygen concentration exerts a major influence over trophoblast activity and, hypoxia inducible factors are proposed to be one of many key regulators of first trimester trophoblast behaviour. We used a global gene expression microarray approach to identify signalling pathways and hypoxia-responsive genes of interest involved in invasion of the first trimester trophoblast cell line HTR8/SVneo under hypoxic conditions where HIF-1 was active. Additionally, first trimester placental samples from different gestational age groups were labelled with anti HIF-1α and HIF-2α to evaluate whether HIFs are differentially expressed and localised across two periods of placental development: (1) early first trimester characterised by hypoxia (6–8 weeks) and (2) late first trimester after initiation of maternal blood flow into the placenta (10–12 weeks). Invasion of HTR8/SVneo was assessed in real-time and was significantly increased in 1% compared with 5% and 21% oxygen and did not differ between 5% and 21% oxygen treatments. Eighty-eight genes were differentially expressed between cells cultured in 1% oxygen (where HIF-1α protein was localised to the nucleus) and 5% oxygen (where HIF-1α was mainly cytoplasmic). 65% of the genes were predicted to contain HIF-1α:HIF-1β transcription factor binding sites. While HIF-2α staining intensity in trophoblasts of late first trimester placenta was higher than early first trimester (+57%) the percentage of positively stained trophoblast nuclei did not differ between the two time points. There was no difference in the expression level of any of the hypoxia responsive genes of interest, IGFBP3, P4HA1, P4HA2, ANGPTL4 and MMP1 between early and late first trimester placenta. While HIF-1α and its downstream targets are clearly induced in HTR8/SVneo during in vitro hypoxic conditions, it appears that hypoxia inducible factors and genes are not altered throughout the first 7–12 weeks of placental development, during which the onset of maternal blood flow to the intervillous space takes place." @default.
• W2174396521 created "2016-06-24" @default.
• W2174396521 creator A5001363437 @default.
• W2174396521 creator A5004355758 @default.
• W2174396521 creator A5040208075 @default.
• W2174396521 creator A5042201449 @default.
• W2174396521 creator A5052339374 @default.
• W2174396521 creator A5057111698 @default.
• W2174396521 creator A5062132866 @default.
• W2174396521 creator A5067238438 @default.
• W2174396521 date "2015-12-01" @default.
• W2174396521 modified "2023-10-16" @default.
• W2174396521 title "Hypoxia induced HIF-1/HIF-2 activity alters trophoblast transcriptional regulation and promotes invasion" @default.
• W2174396521 cites W1499357795 @default.
• W2174396521 cites W1570638334 @default.
• W2174396521 cites W1577577364 @default.
• W2174396521 cites W171819190 @default.
• W2174396521 cites W1749022239 @default.
• W2174396521 cites W1964279292 @default.
• W2174396521 cites W1964516744 @default.
• W2174396521 cites W1965781378 @default.
• W2174396521 cites W1969590867 @default.
• W2174396521 cites W1969672679 @default.
• W2174396521 cites W1971163936 @default.
• W2174396521 cites W1972032694 @default.
• W2174396521 cites W1982658831 @default.
• W2174396521 cites W1990472534 @default.
• W2174396521 cites W1990834080 @default.
• W2174396521 cites W1997113758 @default.
• W2174396521 cites W1998197932 @default.
• W2174396521 cites W1999090873 @default.
• W2174396521 cites W2002014577 @default.
• W2174396521 cites W2002771508 @default.
• W2174396521 cites W2003509389 @default.
• W2174396521 cites W2010386340 @default.
• W2174396521 cites W2015327546 @default.
• W2174396521 cites W2019997266 @default.
• W2174396521 cites W2020049282 @default.
• W2174396521 cites W2022067714 @default.
• W2174396521 cites W2024206845 @default.
• W2174396521 cites W2029425171 @default.
• W2174396521 cites W2032665278 @default.
• W2174396521 cites W2040031153 @default.
• W2174396521 cites W2042624112 @default.
• W2174396521 cites W2042733876 @default.
• W2174396521 cites W2044008105 @default.
• W2174396521 cites W2047408341 @default.
• W2174396521 cites W2050087753 @default.
• W2174396521 cites W2050561859 @default.
• W2174396521 cites W2050651935 @default.
• W2174396521 cites W2061459613 @default.
• W2174396521 cites W2062251724 @default.
• W2174396521 cites W2064178384 @default.
• W2174396521 cites W2066595979 @default.
• W2174396521 cites W2069081088 @default.
• W2174396521 cites W2071143412 @default.
• W2174396521 cites W2072177711 @default.
• W2174396521 cites W2072296588 @default.
• W2174396521 cites W2072661023 @default.
• W2174396521 cites W2073204295 @default.
• W2174396521 cites W2079121632 @default.
• W2174396521 cites W2079773807 @default.
• W2174396521 cites W2080374319 @default.
• W2174396521 cites W2086449036 @default.
• W2174396521 cites W2089437140 @default.
• W2174396521 cites W2094891219 @default.
• W2174396521 cites W2099582683 @default.
• W2174396521 cites W2101084498 @default.
• W2174396521 cites W2106442269 @default.
• W2174396521 cites W2106608030 @default.
• W2174396521 cites W2106782975 @default.
• W2174396521 cites W2107807255 @default.
• W2174396521 cites W2108548188 @default.
• W2174396521 cites W2111984223 @default.
• W2174396521 cites W2123375603 @default.
• W2174396521 cites W2127300943 @default.
• W2174396521 cites W2132156533 @default.
• W2174396521 cites W2134715197 @default.
• W2174396521 cites W2134721852 @default.
• W2174396521 cites W2138581811 @default.
• W2174396521 cites W2138670825 @default.
• W2174396521 cites W2139830524 @default.
• W2174396521 cites W2140607197 @default.
• W2174396521 cites W2142261706 @default.
• W2174396521 cites W2144625075 @default.
• W2174396521 cites W2146799912 @default.
• W2174396521 cites W2149313104 @default.
• W2174396521 cites W2149541458 @default.
• W2174396521 cites W2153800130 @default.
• W2174396521 cites W2158267699 @default.
• W2174396521 cites W2159096710 @default.
• W2174396521 cites W2160782334 @default.
• W2174396521 cites W2300710538 @default.
• W2174396521 cites W4230362449 @default.
• W2174396521 cites W4242075931 @default.
• W2174396521 doi "https://doi.org/10.1016/j.ejcb.2015.10.004" @default.
• W2174396521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26531845" @default.
• W2174396521 hasPublicationYear "2015" @default.

• next