FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2174610287> ?p ?o ?g. }

• W2174610287 endingPage "202" @default.
• W2174610287 startingPage "193" @default.
• W2174610287 abstract "In this work, we present the first XOS degrading glycoside hydrolase from Weissella, WXyn43, a two-domain enzyme from GH43. The gene was amplified from genomic DNA of the XOS utilizing Weissella strain 92, classified under the species-pair Weissella cibaria/W.confusa, and expressed in Escherichia coli. The enzyme is lacking a putative signal peptide and is, from a homology model, shown to be composed of an N-terminal 5-fold β-propeller catalytic domain and a C-terminal β-sandwich domain of unknown function. WXyn43 hydrolyzed short (1-4)-β-D-xylooligosaccharides, with similar kcat/KM for xylobiose (X2) and xylotriose (X3) and clearly lower efficiency in xylotetraose (X4) conversion. WXyn43 displays the highest reported kcat for conversion of X3 (900 s(-1) at 37 °C) and X4 (770 s(-1)), and kcat for hydrolysis of X2 (907 s(-1)) is comparable with or greater than the highest previously reported. The purified enzyme adopted a homotetrameric state in solution, while a truncated form with isolated N-terminal catalytic domain adopted a mixture of oligomeric states and lacked detectable activity. The homology model shows that residues from both domains are involved in monomer-monomer hydrogen bonds, while the bonds creating dimer-dimer interactions only involved residues from the N-terminal domain. Docking of X2 and X3 in the active site shows interactions corresponding to subsites -1 and +1, while presence of a third subsite is unclear, but interactions between a loop and the reducing-end xylose of X3 may be present." @default.
• W2174610287 created "2016-06-24" @default.
• W2174610287 creator A5024003342 @default.
• W2174610287 creator A5033042329 @default.
• W2174610287 creator A5047896793 @default.
• W2174610287 creator A5076080892 @default.
• W2174610287 date "2015-10-22" @default.
• W2174610287 modified "2023-10-17" @default.
• W2174610287 title "Characterization of a family 43 β-xylosidase from the xylooligosaccharide utilizing putative probiotic<i>Weissella</i>sp. strain 92" @default.
• W2174610287 cites W1966014684 @default.
• W2174610287 cites W1968158831 @default.
• W2174610287 cites W1980998830 @default.
• W2174610287 cites W1981375218 @default.
• W2174610287 cites W1983464474 @default.
• W2174610287 cites W1983754267 @default.
• W2174610287 cites W1999369435 @default.
• W2174610287 cites W2008232993 @default.
• W2174610287 cites W2019431437 @default.
• W2174610287 cites W2033829415 @default.
• W2174610287 cites W2046882994 @default.
• W2174610287 cites W2070442553 @default.
• W2174610287 cites W2075344151 @default.
• W2174610287 cites W2083893895 @default.
• W2174610287 cites W2089181947 @default.
• W2174610287 cites W2090810691 @default.
• W2174610287 cites W2110235509 @default.
• W2174610287 cites W2112535784 @default.
• W2174610287 cites W2114089110 @default.
• W2174610287 cites W2124377242 @default.
• W2174610287 cites W2127388955 @default.
• W2174610287 cites W2128873562 @default.
• W2174610287 cites W2129360213 @default.
• W2174610287 cites W2132629607 @default.
• W2174610287 cites W2132794511 @default.
• W2174610287 cites W2156803450 @default.
• W2174610287 cites W2158032510 @default.
• W2174610287 cites W2161129780 @default.
• W2174610287 cites W2328023807 @default.
• W2174610287 cites W2407623182 @default.
• W2174610287 cites W93459794 @default.
• W2174610287 doi "https://doi.org/10.1093/glycob/cwv092" @default.
• W2174610287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4691288" @default.
• W2174610287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26494804" @default.
• W2174610287 hasPublicationYear "2015" @default.
• W2174610287 type Work @default.
• W2174610287 sameAs 2174610287 @default.
• W2174610287 citedByCount "22" @default.
• W2174610287 countsByYear W21746102872016 @default.
• W2174610287 countsByYear W21746102872017 @default.
• W2174610287 countsByYear W21746102872018 @default.
• W2174610287 countsByYear W21746102872019 @default.
• W2174610287 countsByYear W21746102872020 @default.
• W2174610287 countsByYear W21746102872021 @default.
• W2174610287 countsByYear W21746102872022 @default.
• W2174610287 countsByYear W21746102872023 @default.
• W2174610287 crossrefType "journal-article" @default.
• W2174610287 hasAuthorship W2174610287A5024003342 @default.
• W2174610287 hasAuthorship W2174610287A5033042329 @default.
• W2174610287 hasAuthorship W2174610287A5047896793 @default.
• W2174610287 hasAuthorship W2174610287A5076080892 @default.
• W2174610287 hasBestOaLocation W21746102871 @default.
• W2174610287 hasConcept C100544194 @default.
• W2174610287 hasConcept C104317684 @default.
• W2174610287 hasConcept C159110408 @default.
• W2174610287 hasConcept C169627665 @default.
• W2174610287 hasConcept C178790620 @default.
• W2174610287 hasConcept C181199279 @default.
• W2174610287 hasConcept C185592680 @default.
• W2174610287 hasConcept C196032511 @default.
• W2174610287 hasConcept C2775859485 @default.
• W2174610287 hasConcept C2778859111 @default.
• W2174610287 hasConcept C2779546866 @default.
• W2174610287 hasConcept C2779920640 @default.
• W2174610287 hasConcept C2780125375 @default.
• W2174610287 hasConcept C2780206646 @default.
• W2174610287 hasConcept C2780294150 @default.
• W2174610287 hasConcept C41183919 @default.
• W2174610287 hasConcept C41685203 @default.
• W2174610287 hasConcept C547475151 @default.
• W2174610287 hasConcept C55493867 @default.
• W2174610287 hasConcept C56856141 @default.
• W2174610287 hasConcept C71240020 @default.
• W2174610287 hasConcept C71924100 @default.
• W2174610287 hasConcept C94412978 @default.
• W2174610287 hasConceptScore W2174610287C100544194 @default.
• W2174610287 hasConceptScore W2174610287C104317684 @default.
• W2174610287 hasConceptScore W2174610287C159110408 @default.
• W2174610287 hasConceptScore W2174610287C169627665 @default.
• W2174610287 hasConceptScore W2174610287C178790620 @default.
• W2174610287 hasConceptScore W2174610287C181199279 @default.
• W2174610287 hasConceptScore W2174610287C185592680 @default.
• W2174610287 hasConceptScore W2174610287C196032511 @default.
• W2174610287 hasConceptScore W2174610287C2775859485 @default.
• W2174610287 hasConceptScore W2174610287C2778859111 @default.
• W2174610287 hasConceptScore W2174610287C2779546866 @default.
• W2174610287 hasConceptScore W2174610287C2779920640 @default.
• W2174610287 hasConceptScore W2174610287C2780125375 @default.
• W2174610287 hasConceptScore W2174610287C2780206646 @default.

• next