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• W2174658119 abstract "Bacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. In this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. We attempted to enrich infectious bacteriophages from sewage against nosocomial drug-resistant bacterial strains of different medically important species in order to evaluate the probability of discovering novel therapeutic phages. Stability and host-range were determined for the acquired phages. Our results suggest that on-demand isolation of phages is possible against Pseudomonas aeruginosa, Salmonella and extended spectrum beta-lactamase (ESBL) Escherichia coli and Klebsiella pneumoniae. The probability of finding suitable phages was less than 40% against vancomycin resistant Enterococcus (VRE) and Acinetobacter baumannii strains. Furthermore, isolation of new phages against methicillin resistant Staphylococcus aureus (MRSA) strains was found to be very difficult." @default.
• W2174658119 created "2016-06-24" @default.
• W2174658119 creator A5013689373 @default.
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• W2174658119 date "2015-11-13" @default.
• W2174658119 modified "2023-10-15" @default.
• W2174658119 title "On-Demand Isolation of Bacteriophages Against Drug-Resistant Bacteria for Personalized Phage Therapy" @default.
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• W2174658119 doi "https://doi.org/10.3389/fmicb.2015.01271" @default.
• W2174658119 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4643220" @default.
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• W2174658119 hasPublicationYear "2015" @default.
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