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• W2174996751 startingPage "1" @default.
• W2174996751 abstract "The first papers describing the use of insect cell baculoviruses as expression vectors were published in the early 1980’s (Smith et al., 1983; Pennock et al., 1984). Interestingly, as measured by the number of published papers identified using the terms “recombinant and baculovir*” to search the PubMed database, the system had only limited use through 1990 (Fig. 1). One reason for the lack of utilization over this period may have been due to the tedious procedures initially required to identify and isolate recombinant baculoviruses (rBV). The recombination frequency of the original baculovirus expression vector system (BEVS) was very low, less than 1%. This low recombination frequency made it difficult for those unskilled in the art to identify and isolate purified recombinant viruses. In the 1990’s the development of a variety of new transfer plasmids (Jarvis, 1997), linearized, gapped viral DNA’s (Kitts et al., 1990; Kitts and Possee, 1993) and a novel system based on site-specific transposition in E. coli (Luckow et al., 1993) greatly simplified and rapidly expanded the use of the system. In addition, the reagents required for generating recombinant viruses became more widely available through commercial sources." @default.
• W2174996751 created "2016-06-24" @default.
• W2174996751 creator A5000446320 @default.
• W2174996751 creator A5072342779 @default.
• W2174996751 creator A5079039427 @default.
• W2174996751 date "2000-01-01" @default.
• W2174996751 modified "2023-10-17" @default.
• W2174996751 title "Application of Recombinant Baculoviruses in Biopharmaceutical Research" @default.
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