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- W2175316901 abstract "As cell culture provides an isolated system for experimental work, with its improved techniques, the workers have been attracted toward it. In the fields of cytogenetics, radiation biology, virology, developmental biology, biochemistry, and many others, cell culture has made it possible to perform experiments that are impractical or impossible to do in whole organisms—especially where human cells are involved. With time and experience, the workers in each area rapidly reach the point where even the cell culture system begins to look complex and inadequate. The growing cell culture contains cells in all phases of their reproductive cycle, and in each phase the cells exhibit differing sensitivities to radiation, virus infection, enzyme induction, etc. Even if the culture may contain only one cell type, the random distribution of cells in different phases of the reproductive (asynchrony) cycle makes the culture effectively heterogeneous for many kinds of experiments. A variety of approaches have been developed to overcome this problem. The chapter reviews and compares the distinct advantages of synchronized cell cultures." @default.
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- W2175316901 date "1969-01-01" @default.
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- W2175316901 title "Chapter 2 Synchronization Methods for Mammalian Cell Cultures" @default.
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- W2175316901 doi "https://doi.org/10.1016/s0091-679x(08)62113-5" @default.
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