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• W2175442085 abstract "Article1 September 1987free access Engineering herbicide resistance in plants by expression of a detoxifying enzyme M. De Block M. De Block Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author J. Botterman J. Botterman Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author M. Vandewiele M. Vandewiele Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author J. Dockx J. Dockx Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author C. Thoen C. Thoen Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author V. Gosselé V. Gosselé Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author N. Rao Movva N. Rao Movva Biogen S. A., 46, Route des Acacias, CH-1227 Geneva, Switzerland Search for more papers by this author C. Thompson C. Thompson Biogen S. A., 46, Route des Acacias, CH-1227 Geneva, Switzerland Present address: Institut Pasteur, 25 et 28 Rue du Docteur Roux, 75724 Paris F-Cedex 15, France Search for more papers by this author M. Van Montagu M. Van Montagu Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author J. Leemans J. Leemans Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author M. De Block M. De Block Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author J. Botterman J. Botterman Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author M. Vandewiele M. Vandewiele Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author J. Dockx J. Dockx Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author C. Thoen C. Thoen Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author V. Gosselé V. Gosselé Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author N. Rao Movva N. Rao Movva Biogen S. A., 46, Route des Acacias, CH-1227 Geneva, Switzerland Search for more papers by this author C. Thompson C. Thompson Biogen S. A., 46, Route des Acacias, CH-1227 Geneva, Switzerland Present address: Institut Pasteur, 25 et 28 Rue du Docteur Roux, 75724 Paris F-Cedex 15, France Search for more papers by this author M. Van Montagu M. Van Montagu Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author J. Leemans J. Leemans Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium Search for more papers by this author Author Information M. De Block1, J. Botterman1, M. Vandewiele1, J. Dockx1, C. Thoen1, V. Gosselé1, N. Rao Movva2, C. Thompson2, M. Van Montagu1 and J. Leemans1 1Plant Genetic Systems N. V., J. Plateaustraat 22, B-9000 Gent, Belgium 2Biogen S. A., 46, Route des Acacias, CH-1227 Geneva, Switzerland The EMBO Journal (1987)6:2513-2518https://doi.org/10.1002/j.1460-2075.1987.tb02537.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Phosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, encodes a phosphinothricin acetyltransferase (PAT) (see accompanying paper). The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus and transferred to plant cells using Agrobacterium-mediated transformation. PAT was used as a selectable marker in protoplast co-cultivation. The chimeric bar gene was expressed in tobacco, potato and tomato plants. Transgenic plants showed complete resistance towards high doses of the commercial formulations of phosphinothricin and bialaphos. These data present a successful approach to obtain herbicide-resistant plants by detoxification of the herbicide. Previous ArticleNext Article Volume 6Issue 91 September 1987In this issue RelatedDetailsLoading ..." @default.
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