Matches in SemOpenAlex for { <https://semopenalex.org/work/W2175536752> ?p ?o ?g. }
- W2175536752 abstract "In the last decade, a powerful biotechnological tool for the in vivo and in vitro specific labeling of proteins (SNAP-tag™ technology) was proposed as a valid alternative to classical protein-tags (green fluorescent proteins, GFPs). This was made possible by the discovery of the irreversible reaction of the human alkylguanine-DNA-alkyl-transferase (hAGT) in the presence of benzyl-guanine derivatives. However, the mild reaction conditions and the general instability of the mesophilic SNAP-tag™ make this new approach not fully applicable to (hyper-)thermophilic and, in general, extremophilic organisms. Here, we introduce an engineered variant of the thermostable alkylguanine-DNA-alkyl-transferase from the Archaea Sulfolobus solfataricus (SsOGT-H5), which displays a catalytic efficiency comparable to the SNAP-tag™ protein, but showing high intrinsic stability typical of proteins from this organism. The successful heterologous expression obtained in a thermophilic model organism makes SsOGT-H5 a valid candidate as protein-tag for organisms living in extreme environments." @default.
- W2175536752 created "2016-06-24" @default.
- W2175536752 creator A5003339898 @default.
- W2175536752 creator A5005179468 @default.
- W2175536752 creator A5014734647 @default.
- W2175536752 creator A5017132282 @default.
- W2175536752 creator A5045705816 @default.
- W2175536752 creator A5060740762 @default.
- W2175536752 creator A5061989033 @default.
- W2175536752 creator A5070865927 @default.
- W2175536752 creator A5075869335 @default.
- W2175536752 date "2015-12-14" @default.
- W2175536752 modified "2023-10-17" @default.
- W2175536752 title "Erratum to: A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA-alkyl-transferase by protein engineering" @default.
- W2175536752 cites W1970270735 @default.
- W2175536752 cites W1984086177 @default.
- W2175536752 cites W1987533844 @default.
- W2175536752 cites W1996338823 @default.
- W2175536752 cites W2000106654 @default.
- W2175536752 cites W2008466187 @default.
- W2175536752 cites W2016036293 @default.
- W2175536752 cites W2023053556 @default.
- W2175536752 cites W2025515800 @default.
- W2175536752 cites W2030271524 @default.
- W2175536752 cites W2032579724 @default.
- W2175536752 cites W2039692671 @default.
- W2175536752 cites W2041466323 @default.
- W2175536752 cites W2049163206 @default.
- W2175536752 cites W2054851997 @default.
- W2175536752 cites W2056771672 @default.
- W2175536752 cites W2057141116 @default.
- W2175536752 cites W2062623595 @default.
- W2175536752 cites W2068373996 @default.
- W2175536752 cites W2097231721 @default.
- W2175536752 cites W2098672022 @default.
- W2175536752 cites W2111689950 @default.
- W2175536752 cites W2121128278 @default.
- W2175536752 cites W2127068865 @default.
- W2175536752 cites W2136698121 @default.
- W2175536752 cites W2144634347 @default.
- W2175536752 cites W2151822590 @default.
- W2175536752 cites W2164553847 @default.
- W2175536752 cites W2164784743 @default.
- W2175536752 cites W2168455096 @default.
- W2175536752 cites W2169515696 @default.
- W2175536752 cites W2171935374 @default.
- W2175536752 cites W2181324122 @default.
- W2175536752 cites W2412744034 @default.
- W2175536752 cites W3126046906 @default.
- W2175536752 doi "https://doi.org/10.1007/s00792-015-0803-9" @default.
- W2175536752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26666951" @default.
- W2175536752 hasPublicationYear "2015" @default.
- W2175536752 type Work @default.
- W2175536752 sameAs 2175536752 @default.
- W2175536752 citedByCount "9" @default.
- W2175536752 countsByYear W21755367522017 @default.
- W2175536752 countsByYear W21755367522019 @default.
- W2175536752 countsByYear W21755367522022 @default.
- W2175536752 countsByYear W21755367522023 @default.
- W2175536752 crossrefType "journal-article" @default.
- W2175536752 hasAuthorship W2175536752A5003339898 @default.
- W2175536752 hasAuthorship W2175536752A5005179468 @default.
- W2175536752 hasAuthorship W2175536752A5014734647 @default.
- W2175536752 hasAuthorship W2175536752A5017132282 @default.
- W2175536752 hasAuthorship W2175536752A5045705816 @default.
- W2175536752 hasAuthorship W2175536752A5060740762 @default.
- W2175536752 hasAuthorship W2175536752A5061989033 @default.
- W2175536752 hasAuthorship W2175536752A5070865927 @default.
- W2175536752 hasAuthorship W2175536752A5075869335 @default.
- W2175536752 hasBestOaLocation W21755367521 @default.
- W2175536752 hasConcept C104317684 @default.
- W2175536752 hasConcept C123894998 @default.
- W2175536752 hasConcept C147816474 @default.
- W2175536752 hasConcept C156904656 @default.
- W2175536752 hasConcept C181199279 @default.
- W2175536752 hasConcept C185592680 @default.
- W2175536752 hasConcept C2776376580 @default.
- W2175536752 hasConcept C2778644216 @default.
- W2175536752 hasConcept C2780631447 @default.
- W2175536752 hasConcept C27911776 @default.
- W2175536752 hasConcept C40767141 @default.
- W2175536752 hasConcept C550995028 @default.
- W2175536752 hasConcept C552990157 @default.
- W2175536752 hasConcept C55428944 @default.
- W2175536752 hasConcept C55493867 @default.
- W2175536752 hasConcept C70721500 @default.
- W2175536752 hasConcept C86803240 @default.
- W2175536752 hasConceptScore W2175536752C104317684 @default.
- W2175536752 hasConceptScore W2175536752C123894998 @default.
- W2175536752 hasConceptScore W2175536752C147816474 @default.
- W2175536752 hasConceptScore W2175536752C156904656 @default.
- W2175536752 hasConceptScore W2175536752C181199279 @default.
- W2175536752 hasConceptScore W2175536752C185592680 @default.
- W2175536752 hasConceptScore W2175536752C2776376580 @default.
- W2175536752 hasConceptScore W2175536752C2778644216 @default.
- W2175536752 hasConceptScore W2175536752C2780631447 @default.
- W2175536752 hasConceptScore W2175536752C27911776 @default.
- W2175536752 hasConceptScore W2175536752C40767141 @default.
- W2175536752 hasConceptScore W2175536752C550995028 @default.
- W2175536752 hasConceptScore W2175536752C552990157 @default.