Matches in SemOpenAlex for { <https://semopenalex.org/work/W2175637972> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2175637972 endingPage "832" @default.
- W2175637972 startingPage "826" @default.
- W2175637972 abstract "The transforming growth factor‐β/bone morphogenic protein/Smad signaling pathway has been raised as a new and promising therapeutic target of heterotopic ossification, which is mediated by recruitment of transcription coactivator Yes‐associated protein (YAP) to Smad. Here, we described a successful integration of computational modeling and experimental assay to rationally design novel peptide aptamers to disrupt YAP–Smad interaction by targeting YAP WW1 domain. In the protocol, a computational genetic evolution strategy was used to improve a population of potential YAP WW1‐binding peptides generated from the YAP‐recognition site in Smad protein, from which several promising peptides were selected and their affinities toward YAP WW1 domain were determined using binding assay. In addition, a high‐activity peptide was further optimized based on its complex structure with YAP WW1 domain to derive a number of derivative peptides with higher binding potency to the domain. We also found that a strong YAP WW1 binder should have a negatively charged N‐terminus, a positively charged C‐terminus and a nonpolar core to match the electrostatic distribution pattern in peptide‐binding pocket of YAP WW1 domain, which may also form additional nonbonded interactions such as hydrogen bond, salt bridge and π–π stacking to confer stability and specificity for the domain–peptide recognition. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd." @default.
- W2175637972 created "2016-06-24" @default.
- W2175637972 creator A5001639742 @default.
- W2175637972 creator A5020419415 @default.
- W2175637972 creator A5048071821 @default.
- W2175637972 creator A5063975484 @default.
- W2175637972 date "2015-10-05" @default.
- W2175637972 modified "2023-10-16" @default.
- W2175637972 title "Rational design of YAP WW1 domain-binding peptides to target TGFβ/BMP/Smad-YAP interaction in heterotopic ossification" @default.
- W2175637972 cites W1851343525 @default.
- W2175637972 cites W1908759766 @default.
- W2175637972 cites W1975580333 @default.
- W2175637972 cites W1976499671 @default.
- W2175637972 cites W1996070102 @default.
- W2175637972 cites W1997627388 @default.
- W2175637972 cites W2015403140 @default.
- W2175637972 cites W2019777614 @default.
- W2175637972 cites W2030352395 @default.
- W2175637972 cites W2034469841 @default.
- W2175637972 cites W2046270293 @default.
- W2175637972 cites W2052046618 @default.
- W2175637972 cites W2053302020 @default.
- W2175637972 cites W2069123478 @default.
- W2175637972 cites W2081912151 @default.
- W2175637972 cites W2098631451 @default.
- W2175637972 cites W2098888753 @default.
- W2175637972 cites W2120216126 @default.
- W2175637972 cites W2130479394 @default.
- W2175637972 cites W2161932029 @default.
- W2175637972 cites W2172270516 @default.
- W2175637972 doi "https://doi.org/10.1002/psc.2824" @default.
- W2175637972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26435515" @default.
- W2175637972 hasPublicationYear "2015" @default.
- W2175637972 type Work @default.
- W2175637972 sameAs 2175637972 @default.
- W2175637972 citedByCount "18" @default.
- W2175637972 countsByYear W21756379722016 @default.
- W2175637972 countsByYear W21756379722017 @default.
- W2175637972 countsByYear W21756379722018 @default.
- W2175637972 countsByYear W21756379722019 @default.
- W2175637972 countsByYear W21756379722020 @default.
- W2175637972 countsByYear W21756379722021 @default.
- W2175637972 crossrefType "journal-article" @default.
- W2175637972 hasAuthorship W2175637972A5001639742 @default.
- W2175637972 hasAuthorship W2175637972A5020419415 @default.
- W2175637972 hasAuthorship W2175637972A5048071821 @default.
- W2175637972 hasAuthorship W2175637972A5063975484 @default.
- W2175637972 hasConcept C118131993 @default.
- W2175637972 hasConcept C12554922 @default.
- W2175637972 hasConcept C192562407 @default.
- W2175637972 hasConcept C2779281246 @default.
- W2175637972 hasConcept C2781080636 @default.
- W2175637972 hasConcept C55493867 @default.
- W2175637972 hasConcept C86803240 @default.
- W2175637972 hasConcept C95444343 @default.
- W2175637972 hasConceptScore W2175637972C118131993 @default.
- W2175637972 hasConceptScore W2175637972C12554922 @default.
- W2175637972 hasConceptScore W2175637972C192562407 @default.
- W2175637972 hasConceptScore W2175637972C2779281246 @default.
- W2175637972 hasConceptScore W2175637972C2781080636 @default.
- W2175637972 hasConceptScore W2175637972C55493867 @default.
- W2175637972 hasConceptScore W2175637972C86803240 @default.
- W2175637972 hasConceptScore W2175637972C95444343 @default.
- W2175637972 hasIssue "11" @default.
- W2175637972 hasLocation W21756379721 @default.
- W2175637972 hasOpenAccess W2175637972 @default.
- W2175637972 hasPrimaryLocation W21756379721 @default.
- W2175637972 hasRelatedWork W2059088542 @default.
- W2175637972 hasRelatedWork W2376327684 @default.
- W2175637972 hasRelatedWork W2378469710 @default.
- W2175637972 hasRelatedWork W2389979363 @default.
- W2175637972 hasRelatedWork W2392362949 @default.
- W2175637972 hasRelatedWork W2392720586 @default.
- W2175637972 hasRelatedWork W2979862721 @default.
- W2175637972 hasRelatedWork W4200539503 @default.
- W2175637972 hasRelatedWork W4285386300 @default.
- W2175637972 hasRelatedWork W4310977130 @default.
- W2175637972 hasVolume "21" @default.
- W2175637972 isParatext "false" @default.
- W2175637972 isRetracted "false" @default.
- W2175637972 magId "2175637972" @default.
- W2175637972 workType "article" @default.