Matches in SemOpenAlex for { <https://semopenalex.org/work/W2176166968> ?p ?o ?g. }
- W2176166968 abstract "Abstract Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute tissue-specific phenotypes is currently unknown. Here we show that hPSC-KCs self-organize into kidney organoids that functionally recapitulate tissue-specific epithelial physiology, including disease phenotypes after genome editing. In three-dimensional cultures, epiblast-stage hPSCs form spheroids surrounding hollow, amniotic-like cavities. GSK3β inhibition differentiates spheroids into segmented, nephron-like kidney organoids containing cell populations with characteristics of proximal tubules, podocytes and endothelium. Tubules accumulate dextran and methotrexate transport cargoes, and express kidney injury molecule-1 after nephrotoxic chemical injury. CRISPR/Cas9 knockout of podocalyxin causes junctional organization defects in podocyte-like cells. Knockout of the polycystic kidney disease genes PKD1 or PKD2 induces cyst formation from kidney tubules. All of these functional phenotypes are distinct from effects in epiblast spheroids, indicating that they are tissue specific. Our findings establish a reproducible, versatile three-dimensional framework for human epithelial disease modelling and regenerative medicine applications." @default.
- W2176166968 created "2016-06-24" @default.
- W2176166968 creator A5002046839 @default.
- W2176166968 creator A5004746201 @default.
- W2176166968 creator A5007980266 @default.
- W2176166968 creator A5012007728 @default.
- W2176166968 creator A5023720090 @default.
- W2176166968 creator A5023793227 @default.
- W2176166968 creator A5026897418 @default.
- W2176166968 creator A5032821089 @default.
- W2176166968 creator A5033808062 @default.
- W2176166968 creator A5040568538 @default.
- W2176166968 creator A5040943880 @default.
- W2176166968 creator A5041571580 @default.
- W2176166968 creator A5055337266 @default.
- W2176166968 creator A5055614926 @default.
- W2176166968 creator A5056992469 @default.
- W2176166968 creator A5078965237 @default.
- W2176166968 creator A5087807841 @default.
- W2176166968 creator A5088299046 @default.
- W2176166968 creator A5088781783 @default.
- W2176166968 creator A5089043211 @default.
- W2176166968 creator A5089781525 @default.
- W2176166968 date "2015-10-23" @default.
- W2176166968 modified "2023-10-17" @default.
- W2176166968 title "Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids" @default.
- W2176166968 cites W140105264 @default.
- W2176166968 cites W1574613092 @default.
- W2176166968 cites W1599568627 @default.
- W2176166968 cites W1602471950 @default.
- W2176166968 cites W1858571698 @default.
- W2176166968 cites W1967800133 @default.
- W2176166968 cites W1969366572 @default.
- W2176166968 cites W1970604167 @default.
- W2176166968 cites W1980020302 @default.
- W2176166968 cites W1982099181 @default.
- W2176166968 cites W1985195015 @default.
- W2176166968 cites W2000529605 @default.
- W2176166968 cites W2000714884 @default.
- W2176166968 cites W2003171404 @default.
- W2176166968 cites W2010252933 @default.
- W2176166968 cites W2012034410 @default.
- W2176166968 cites W2023034253 @default.
- W2176166968 cites W2024398044 @default.
- W2176166968 cites W2025175358 @default.
- W2176166968 cites W2026527439 @default.
- W2176166968 cites W2029706942 @default.
- W2176166968 cites W2037982474 @default.
- W2176166968 cites W2040067847 @default.
- W2176166968 cites W2044120077 @default.
- W2176166968 cites W2045421375 @default.
- W2176166968 cites W2051272245 @default.
- W2176166968 cites W2058521801 @default.
- W2176166968 cites W2059925153 @default.
- W2176166968 cites W2060837465 @default.
- W2176166968 cites W2069354876 @default.
- W2176166968 cites W2078112396 @default.
- W2176166968 cites W2080853555 @default.
- W2176166968 cites W2083599207 @default.
- W2176166968 cites W2088811685 @default.
- W2176166968 cites W2090048264 @default.
- W2176166968 cites W2090260381 @default.
- W2176166968 cites W2093401766 @default.
- W2176166968 cites W2099231076 @default.
- W2176166968 cites W2101140701 @default.
- W2176166968 cites W2103565029 @default.
- W2176166968 cites W2106127625 @default.
- W2176166968 cites W2107175020 @default.
- W2176166968 cites W2109120895 @default.
- W2176166968 cites W2112965812 @default.
- W2176166968 cites W2116228354 @default.
- W2176166968 cites W2133691034 @default.
- W2176166968 cites W2138977668 @default.
- W2176166968 cites W2152793450 @default.
- W2176166968 cites W2153818720 @default.
- W2176166968 cites W2154450105 @default.
- W2176166968 cites W2157034440 @default.
- W2176166968 cites W2157645703 @default.
- W2176166968 cites W2168450345 @default.
- W2176166968 cites W2266096083 @default.
- W2176166968 doi "https://doi.org/10.1038/ncomms9715" @default.
- W2176166968 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4620584" @default.
- W2176166968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26493500" @default.
- W2176166968 hasPublicationYear "2015" @default.
- W2176166968 type Work @default.
- W2176166968 sameAs 2176166968 @default.
- W2176166968 citedByCount "544" @default.
- W2176166968 countsByYear W21761669682015 @default.
- W2176166968 countsByYear W21761669682016 @default.
- W2176166968 countsByYear W21761669682017 @default.
- W2176166968 countsByYear W21761669682018 @default.
- W2176166968 countsByYear W21761669682019 @default.
- W2176166968 countsByYear W21761669682020 @default.
- W2176166968 countsByYear W21761669682021 @default.
- W2176166968 countsByYear W21761669682022 @default.
- W2176166968 countsByYear W21761669682023 @default.
- W2176166968 crossrefType "journal-article" @default.
- W2176166968 hasAuthorship W2176166968A5002046839 @default.
- W2176166968 hasAuthorship W2176166968A5004746201 @default.
- W2176166968 hasAuthorship W2176166968A5007980266 @default.