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- W2176965346 abstract "A novel actinomycete, designated strain NEAU-A-2 T , was isolated from the rhizosphere soil of Sansevieria trifasciata Prain collected from Heilongjiang province, north-east China. The taxonomic status of this organism was established using a polyphasic approach. The isolate formed irregular sporangia containing motile spores on the substrate mycelium. The whole-cell sugars were xylose and galactose. The predominant menaquinones were MK-9(H 10 ), MK-9(H 2 ), MK-10(H 2 ) and MK-10(H 4 ). The major fatty acids were iso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 15 : 0 . The polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and an unidentified glycolipid. 16S rRNA gene sequence similarity studies showed that strain NEAU-A-2 T belongs to the genus Actinoplanes with the highest sequence similarities to Actinoplanes globisporus NBRC 13912 T (97.7 % 16S rRNA gene sequence similarity), Actinoplanes ferrugineus IMSNU 22125 T (97.5 %), Actinoplanes toevensis MN07-A0368 T (97.2 %) and Actinoplanes rishiriensis NBRC 108556 T (97.2 %); similarities to type strains of other species of this genus were < 97 %. Two tree-making algorithms showed that strain NEAU-A-2 T formed a distinct clade with A. globisporus NBRC 13912 T and A. rishiriensis NBRC 108556 T . However, low DNA–DNA relatedness values allowed the isolate to be differentiated from the above-mentioned two species of the genus Actinoplanes . Moreover, strain NEAU-A-2 T could also be distinguished from the most closely related species by morphological and physiological characteristics. Therefore, in conclusion, isolate NEAU-A-2 T represents a novel species of the genus Actinoplanes , for which the name Actinoplanes rhizophilus sp. nov. is proposed. The type strain is NEAU-A-2 T ( = CGMCC 4.7133 T = DSM 46672 T )." @default.
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- W2176965346 date "2015-12-01" @default.
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- W2176965346 title "Actinoplanes rhizophilus sp. nov., an actinomycete isolated from the rhizosphere of Sansevieria trifasciata Prain" @default.
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- W2176965346 doi "https://doi.org/10.1099/ijsem.0.000646" @default.
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