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• W2178041119 abstract "Electron cryomicroscopy (cryo-EM) allows structure determination of a wide range of biological molecules and specimens. All-gold supports improve cryo-EM images by reducing radiation-induced motion and image blurring. Here we compare the mechanical and electrical properties of all-gold supports to amorphous carbon foils. Gold supports are more conductive, and have suspended foils that are not compressed by differential contraction when cooled to liquid nitrogen temperatures. These measurements show how the choice of support material and geometry can reduce specimen movement by more than an order of magnitude during low-dose imaging. We provide methods for fabrication of all-gold supports and preparation of vitrified specimens. We also analyse illumination geometry for optimal collection of high resolution, low-dose data. Together, the support structures and methods herein can improve the resolution and quality of images from any electron cryomicroscope." @default.
• W2178041119 created "2016-06-24" @default.
• W2178041119 creator A5026689567 @default.
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• W2178041119 date "2016-01-01" @default.
• W2178041119 modified "2023-10-17" @default.
• W2178041119 title "Ultrastable gold substrates: Properties of a support for high-resolution electron cryomicroscopy of biological specimens" @default.
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• W2178041119 doi "https://doi.org/10.1016/j.jsb.2015.11.006" @default.
• W2178041119 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4711342" @default.
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