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• W2178171385 abstract "Uncoordinated-5 homolog B receptor (UNC5B) was first found to mediate neural chemorepulsive effects by binding to its ligand netrin-1 in the nervous system. Newer evidence indicated that UNC5B also has functions outside the nervous system. In this study, we report on the generation of a monoclonal antibody specific to the outer-membrane immunoglobulin-like domains of UNC5B using the hybridoma technique. Western blot, immunofluorescence, and flow cytometry analyses showed that the antibody specifically bound to UNC5B protein. Interestingly, the antibody blocked the Netrin-1-induced inhibitory effect on the mobility of melanoma A375 cells by wound healing assay and transwell migration assay, whereas it had no effects on cell proliferation measured by CCK-8 assay. Thus, the functional antibody may provide a useful tool for the study of UNC5B expression profiles and functions outside the nervous system." @default.
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• W2178171385 date "2014-08-01" @default.
• W2178171385 modified "2023-09-26" @default.
• W2178171385 title "An Uncoordinated-5 Homolog B Receptor Monoclonal Antibody Regulates A375 Melanoma Cell Migration" @default.
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• W2178171385 doi "https://doi.org/10.1089/mab.2013.0077" @default.
• W2178171385 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4151061" @default.
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