FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2178225519> ?p ?o ?g. }

• W2178225519 endingPage "73" @default.
• W2178225519 startingPage "666" @default.
• W2178225519 abstract "Phencyclidine (PCP) receptors have been solubilized from rat forebrain membranes with the zwitterionic detergent 3-(3-cholamidopropyl)dimethylammonio-1-propanesulfonate. Specific binding of the potent PCP receptor ligands [3H]thienyl-phencyclidine (TCP) and [3H]MK-801 was restored by incorporating extracted membrane protein into lipid vesicles prepared from a total brain lipid extract. A nearly quantitative recovery of solubilized receptor activity was achieved; this was dependent upon both the concentration of detergent used during membrane solubilization and the concentration of added lipid used during the reconstitution. The single, saturable, binding site measured for both [3H]TCP and [3H]MK-801 in solubilized and reconstituted preparations exhibited properties similar to those of the high affinity PCP binding site labeled by these ligands in brain membranes. The ability of ligands selective for this site (MK-801, TCP, and dexoxadrol) to competitively displace specific [3H]TCP binding was retained after solubilization and reconstitution, although IC50 values measured for these ligands were shifted to higher concentrations. Levoxadrol and haloperidol were ineffective at displacing the radioligand binding in both membrane and vesicle preparations. The additive and dose-dependent ability of glutamate and glycine to enhance [3H]TCP binding to the solubilized/reconstituted receptor further suggests that a direct interaction with the N-methyl-D-aspartate receptor/ion channel complex has been preserved in the vesicle preparations. The photoaffinity labeling of two polypeptides (Mr 98,000 and 59,000) by azido-[3H]PCP was demonstrated in the vesicle preparations; this was largely prevented by competitive displacement of the radioligand with PCP before photolysis. These results establish both an essential lipid dependency and polypeptide composition for the high affinity, haloperidol-insensitive, PCP receptor in brain." @default.
• W2178225519 created "2016-06-24" @default.
• W2178225519 creator A5001318454 @default.
• W2178225519 creator A5052806674 @default.
• W2178225519 creator A5060379349 @default.
• W2178225519 creator A5062028211 @default.
• W2178225519 creator A5083113704 @default.
• W2178225519 date "1991-11-01" @default.
• W2178225519 modified "2023-09-26" @default.
• W2178225519 title "High efficiency reconstitution of a phencyclidine/MK-801 receptor binding site solubilized from rat forebrain membranes." @default.
• W2178225519 cites W1561414505 @default.
• W2178225519 cites W1633926447 @default.
• W2178225519 cites W1773332605 @default.
• W2178225519 cites W1775749144 @default.
• W2178225519 cites W1900414416 @default.
• W2178225519 cites W1973354899 @default.
• W2178225519 cites W1975679961 @default.
• W2178225519 cites W1975910984 @default.
• W2178225519 cites W1980688136 @default.
• W2178225519 cites W1983855603 @default.
• W2178225519 cites W1985961951 @default.
• W2178225519 cites W1986233453 @default.
• W2178225519 cites W1987258940 @default.
• W2178225519 cites W2008670721 @default.
• W2178225519 cites W2013067874 @default.
• W2178225519 cites W2029798646 @default.
• W2178225519 cites W2036209909 @default.
• W2178225519 cites W2043813981 @default.
• W2178225519 cites W2044488172 @default.
• W2178225519 cites W2045300940 @default.
• W2178225519 cites W2048152070 @default.
• W2178225519 cites W2050391960 @default.
• W2178225519 cites W2063001012 @default.
• W2178225519 cites W2070039760 @default.
• W2178225519 cites W2070182120 @default.
• W2178225519 cites W2076346801 @default.
• W2178225519 cites W2084536694 @default.
• W2178225519 cites W2102131116 @default.
• W2178225519 cites W2106625755 @default.
• W2178225519 cites W2146916230 @default.
• W2178225519 cites W2155760129 @default.
• W2178225519 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1658603" @default.
• W2178225519 hasPublicationYear "1991" @default.
• W2178225519 type Work @default.
• W2178225519 sameAs 2178225519 @default.
• W2178225519 citedByCount "0" @default.
• W2178225519 crossrefType "journal-article" @default.
• W2178225519 hasAuthorship W2178225519A5001318454 @default.
• W2178225519 hasAuthorship W2178225519A5052806674 @default.
• W2178225519 hasAuthorship W2178225519A5060379349 @default.
• W2178225519 hasAuthorship W2178225519A5062028211 @default.
• W2178225519 hasAuthorship W2178225519A5083113704 @default.
• W2178225519 hasConcept C107824862 @default.
• W2178225519 hasConcept C12554922 @default.
• W2178225519 hasConcept C130316041 @default.
• W2178225519 hasConcept C169760540 @default.
• W2178225519 hasConcept C170493617 @default.
• W2178225519 hasConcept C185592680 @default.
• W2178225519 hasConcept C2778581200 @default.
• W2178225519 hasConcept C2778851735 @default.
• W2178225519 hasConcept C2779715522 @default.
• W2178225519 hasConcept C41625074 @default.
• W2178225519 hasConcept C529278444 @default.
• W2178225519 hasConcept C55493867 @default.
• W2178225519 hasConcept C67018056 @default.
• W2178225519 hasConcept C67847695 @default.
• W2178225519 hasConcept C86803240 @default.
• W2178225519 hasConceptScore W2178225519C107824862 @default.
• W2178225519 hasConceptScore W2178225519C12554922 @default.
• W2178225519 hasConceptScore W2178225519C130316041 @default.
• W2178225519 hasConceptScore W2178225519C169760540 @default.
• W2178225519 hasConceptScore W2178225519C170493617 @default.
• W2178225519 hasConceptScore W2178225519C185592680 @default.
• W2178225519 hasConceptScore W2178225519C2778581200 @default.
• W2178225519 hasConceptScore W2178225519C2778851735 @default.
• W2178225519 hasConceptScore W2178225519C2779715522 @default.
• W2178225519 hasConceptScore W2178225519C41625074 @default.
• W2178225519 hasConceptScore W2178225519C529278444 @default.
• W2178225519 hasConceptScore W2178225519C55493867 @default.
• W2178225519 hasConceptScore W2178225519C67018056 @default.
• W2178225519 hasConceptScore W2178225519C67847695 @default.
• W2178225519 hasConceptScore W2178225519C86803240 @default.
• W2178225519 hasIssue "5" @default.
• W2178225519 hasLocation W21782255191 @default.
• W2178225519 hasOpenAccess W2178225519 @default.
• W2178225519 hasPrimaryLocation W21782255191 @default.
• W2178225519 hasRelatedWork W1025998060 @default.
• W2178225519 hasRelatedWork W2004105983 @default.
• W2178225519 hasRelatedWork W2006024947 @default.
• W2178225519 hasRelatedWork W2039138016 @default.
• W2178225519 hasRelatedWork W2044488172 @default.
• W2178225519 hasRelatedWork W2121971847 @default.
• W2178225519 hasRelatedWork W2178225519 @default.
• W2178225519 hasRelatedWork W2436144291 @default.
• W2178225519 hasRelatedWork W2467538728 @default.
• W2178225519 hasRelatedWork W3208844945 @default.
• W2178225519 hasVolume "40" @default.
• W2178225519 isParatext "false" @default.

• next