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- W2179290168 abstract "Objective To establish a method of isolation and culture of mesenchymal stem cells(MSCs) from miniswine bone marrow. Method MSCs were isolated from bone marrow by extracting from the posterior superior iliac spine of miniswines and purified by centrifuge and then cultured in single layer in vitro. MSCs quality were evaluated with morphology.Result Survival MSCs appeared morphologically as fusiform and polygon or star in primary and first cultures. All the cultured cells appeared morphologically as fusiform and fibroblast cell-shaped; their length/width ratio was (2~3):1 from second culture. They were confluent after plating for 8~10 days and showed active proliferation in vitro after passage cultures.Conclusion The MSCs isolated from miniswine bone marrow can be cultured stably for long period in vitro. The establishment of isolating and culturing MSCs of miniswines provided a useful animal model for basic study and tissue engineering." @default.
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- W2179290168 date "2010-01-01" @default.
- W2179290168 modified "2023-09-27" @default.
- W2179290168 title "Isolation and culture of mesenchymal stem cells from miniswine bone marrow" @default.
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