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- W2179661424 abstract "Although heat shock proteins are thought to function primarily as intracellular chaperones, the release and potential extracellular functions of heat shock proteins have been the focus of an increasing number of studies. Our particular interest is HspB1 (Hsp25/27) and as astrocytes are an in vivo source of HspB1 it is a reasonable possibility they could release HspB1 in response to local stresses. Using primary cultures of rat cortical astrocytes, we investigated the extracellular release of HspB1 with exposure to amyloid-β (Aβ). In order to assess potential mechanisms of release, we cotreated the cells with compounds that can modulate protein secretion including Brefeldin A, Methyl β-cyclodextrin, and MAP kinase inhibitors. Exposure to Aβ (0.1, 1.0, 2.0 μM) for 24–48 h resulted in a selective release of HspB1 that was insensitive to BFA treatment; none of the other inhibitors had any detectable influence. Protease protection assays indicated that some of the released HspB1 was associated with a membrane bound fraction, and analysis of exosomal preparations indicated the presence of HspB1 in exosomes. Finally, immunoprecipitation experiments demonstrated that the extracellular HspB1 was able to interact with extracellular Aβ. In summary, Aβ can stimulate release of HspB1 from astrocytes, this release is insensitive to Golgi or lipid raft disruption, and HspB1 can be found either free in the medium or associated with exosomes. This release suggests that there is a potential for extracellular HspB1 to be able to bind and sequester extracellular Aβ." @default.
- W2179661424 created "2016-06-24" @default.
- W2179661424 creator A5015065243 @default.
- W2179661424 creator A5069017297 @default.
- W2179661424 creator A5071875389 @default.
- W2179661424 date "2015-11-03" @default.
- W2179661424 modified "2023-09-30" @default.
- W2179661424 title "Astrocytes Release HspB1 in Response to Amyloid-β Exposure in vitro" @default.
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- W2179661424 doi "https://doi.org/10.3233/jad-150317" @default.
- W2179661424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26444769" @default.
- W2179661424 hasPublicationYear "2015" @default.
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