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• W2179836698 abstract "Analysis of single cells in their native environment is a powerful method to address key questions in developmental systems biology. Confocal microscopy imaging of intact tissues, followed by automatic image segmentation, provides a means to conduct cytometric studies while at the same time preserving crucial information about the spatial organization of the tissue and morphological features of the cells. This technique is rapidly evolving but is still not in widespread use among research groups that do not specialize in technique development, perhaps in part for lack of tools that automate repetitive tasks while allowing experts to make the best use of their time in injecting their domain-specific knowledge. Here we focus on a well-established stem cell model system, the C. elegans gonad, as well as on two other model systems widely used to study cell fate specification and morphogenesis: the pre-implantation mouse embryo and the developing mouse olfactory epithelium. We report a pipeline that integrates machine-learning-based cell detection, fast human-in-the-loop curation of these detections, and running of active contours seeded from detections to segment cells. The procedure can be bootstrapped by a small number of manual detections, and outperforms alternative pieces of software we benchmarked on C. elegans gonad datasets. Using cell segmentations to quantify fluorescence contents, we report previously-uncharacterized cell behaviors in the model systems we used. We further show how cell morphological features can be used to identify cell cycle phase; this provides a basis for future tools that will streamline cell cycle experiments by minimizing the need for exogenous cell cycle phase labels. High-throughput 3D segmentation makes it possible to extract rich information from images that are routinely acquired by biologists, and provides insights — in particular with respect to the cell cycle — that would be difficult to derive otherwise." @default.
• W2179836698 created "2016-06-24" @default.
• W2179836698 creator A5014683663 @default.
• W2179836698 creator A5020125811 @default.
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• W2179836698 creator A5057933835 @default.
• W2179836698 creator A5070023972 @default.
• W2179836698 creator A5080159006 @default.
• W2179836698 date "2015-11-25" @default.
• W2179836698 modified "2023-10-14" @default.
• W2179836698 title "Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images" @default.
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