FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2180284899> ?p ?o ?g. }

• W2180284899 endingPage "1258" @default.
• W2180284899 startingPage "1247" @default.
• W2180284899 abstract "A large number of polymorphic simple sequence repeats (SSRs) or microsatellites are needed to develop a genetic map for shrimp. However, developing an SSR map is very time-consuming, expensive, and most SSRs are not specifically linked to gene loci of immediate interest. We report here on our strategy to develop polymorphic markers using expressed sequence tags (ESTs) by designing primers flanking single or multiple SSRs with three or more repeats. A subtracted cDNA library was prepared using RNA from specific pathogen-free (SPF) Litopenaeus vannamei juveniles (∼1 g) collected before (0) and after (48 h) inoculation with the China isolate of white spot syndrome virus (WSSV). A total of 224 clones were sequenced, 194 of which were useful for homology comparisons against annotated genes in NCBI nonredundant (nr) and protein databases, providing 179 sequences encoded by nuclear DNA, 4 mitochondrial DNA, and 11 were similar to portions of WSSV genome. The nuclear sequences clustered in 43 groups, 11 of which were homologous to various ESTs of unknown function, 4 had no homology to any sequence, and 28 showed similarities to known genes of invertebrates and vertebrates, representatives of cellular metabolic processes such as calcium ion balance, cytoskeleton mRNAs, and protein synthesis. A few sequences were homologous to immune system-related (allergens) genes and two were similar to motifs of the sex-lethal gene of Drosophila. A large number of EST sequences were similar to domains of the EF-hand superfamily (Ca2 binding motif and FRQ protein domain of myosin light chains). Single or multiple SSRs with three or more repeats were found in approximately 61% of the 179 nuclear sequences. Primer sets were designed from 28 sequences representing 19 known or putative genes and tested for polymorphism (EST-SSR marker) in a small test panel containing 16 individuals. Ten (53%) of the 19 putative or unknown function genes were polymorphic, 4 monomorphic, and 3 either failed to satisfactorily amplify genomic DNA or the allele amplification conditions need to be further optimized. Five polymorphic ESTs were genotyped with the entire reference mapping family, two of them (actin, accession #CX535973 and shrimp allergen arginine kinase, accession #CX535999) did not amplify with all offspring of the IRMF panel suggesting presence of null alleles, and three of them amplified in most of the IRMF offspring and were used for linkage analysis. EF-hand motif of myosin light chain (accession #CX535935) was placed in ShrimpMap's linkage group 7, whereas ribosomal protein S5 (accession #CX535957) and troponin I (accession #CX535976) remained unassigned. Results indicate that (a) a large number of ESTs isolated from this cDNA library are similar to cytoskeleton mRNAs and may reflect a normal pathway of the cellular response after im infection with WSSV, and (b) primers flanking single or multiple SSRs with three or more repeats from shrimp ESTs could be an efficient approach to develop polymorphic markers useful for linkage mapping. Work is underway to map additional SSR-containing ESTs from this and other cDNA libraries as a plausible strategy to increase marker density in ShrimpMap." @default.
• W2180284899 created "2016-06-24" @default.
• W2180284899 creator A5016109383 @default.
• W2180284899 creator A5018099072 @default.
• W2180284899 creator A5026452633 @default.
• W2180284899 creator A5028930141 @default.
• W2180284899 creator A5047330516 @default.
• W2180284899 creator A5060860686 @default.
• W2180284899 date "2007-12-01" @default.
• W2180284899 modified "2023-10-17" @default.
• W2180284899 title "CHARACTERIZATION AND MAPPING OF EXPRESSED SEQUENCE TAGS ISOLATED FROM A SUBTRACTED CDNA LIBRARY OF LITOPENAEUS VANNAMEI INJECTED WITH WHITE SPOT SYNDROME VIRUS" @default.
• W2180284899 cites W1603584852 @default.
• W2180284899 cites W1610026461 @default.
• W2180284899 cites W1672677726 @default.
• W2180284899 cites W1676316327 @default.
• W2180284899 cites W1877845240 @default.
• W2180284899 cites W1931007673 @default.
• W2180284899 cites W1965514944 @default.
• W2180284899 cites W1971767753 @default.
• W2180284899 cites W1979123585 @default.
• W2180284899 cites W1980042844 @default.
• W2180284899 cites W1988181658 @default.
• W2180284899 cites W1989137506 @default.
• W2180284899 cites W2000033799 @default.
• W2180284899 cites W2011159897 @default.
• W2180284899 cites W2013152250 @default.
• W2180284899 cites W2015941374 @default.
• W2180284899 cites W2027016802 @default.
• W2180284899 cites W2028622454 @default.
• W2180284899 cites W2037576312 @default.
• W2180284899 cites W2039485560 @default.
• W2180284899 cites W2040808444 @default.
• W2180284899 cites W2041212846 @default.
• W2180284899 cites W2045487737 @default.
• W2180284899 cites W2047081355 @default.
• W2180284899 cites W2049405996 @default.
• W2180284899 cites W2049910809 @default.
• W2180284899 cites W2051376474 @default.
• W2180284899 cites W2056496485 @default.
• W2180284899 cites W2058591068 @default.
• W2180284899 cites W2060381779 @default.
• W2180284899 cites W2061441318 @default.
• W2180284899 cites W2062320783 @default.
• W2180284899 cites W2063933421 @default.
• W2180284899 cites W2064671445 @default.
• W2180284899 cites W2066295351 @default.
• W2180284899 cites W2066307344 @default.
• W2180284899 cites W2067056720 @default.
• W2180284899 cites W2067779578 @default.
• W2180284899 cites W2068894766 @default.
• W2180284899 cites W2072528438 @default.
• W2180284899 cites W2073994293 @default.
• W2180284899 cites W2081604176 @default.
• W2180284899 cites W2081923026 @default.
• W2180284899 cites W2090907031 @default.
• W2180284899 cites W2099466736 @default.
• W2180284899 cites W2099552271 @default.
• W2180284899 cites W2101166451 @default.
• W2180284899 cites W2101943680 @default.
• W2180284899 cites W2107792141 @default.
• W2180284899 cites W2117926042 @default.
• W2180284899 cites W2122286760 @default.
• W2180284899 cites W2127028479 @default.
• W2180284899 cites W2130417300 @default.
• W2180284899 cites W2135250008 @default.
• W2180284899 cites W2136304443 @default.
• W2180284899 cites W2138100428 @default.
• W2180284899 cites W2138716304 @default.
• W2180284899 cites W2139148987 @default.
• W2180284899 cites W2139642454 @default.
• W2180284899 cites W2165323295 @default.
• W2180284899 cites W2168141716 @default.
• W2180284899 doi "https://doi.org/10.2983/0730-8000(2007)26[1247:camoes]2.0.co;2" @default.
• W2180284899 hasPublicationYear "2007" @default.
• W2180284899 type Work @default.
• W2180284899 sameAs 2180284899 @default.
• W2180284899 citedByCount "6" @default.
• W2180284899 countsByYear W21802848992019 @default.
• W2180284899 crossrefType "journal-article" @default.
• W2180284899 hasAuthorship W2180284899A5016109383 @default.
• W2180284899 hasAuthorship W2180284899A5018099072 @default.
• W2180284899 hasAuthorship W2180284899A5026452633 @default.
• W2180284899 hasAuthorship W2180284899A5028930141 @default.
• W2180284899 hasAuthorship W2180284899A5047330516 @default.
• W2180284899 hasAuthorship W2180284899A5060860686 @default.
• W2180284899 hasConcept C104317684 @default.
• W2180284899 hasConcept C141231307 @default.
• W2180284899 hasConcept C149034497 @default.
• W2180284899 hasConcept C153911025 @default.
• W2180284899 hasConcept C165525559 @default.
• W2180284899 hasConcept C17757408 @default.
• W2180284899 hasConcept C187882448 @default.
• W2180284899 hasConcept C189482833 @default.
• W2180284899 hasConcept C2779771762 @default.
• W2180284899 hasConcept C54355233 @default.
• W2180284899 hasConcept C61053724 @default.
• W2180284899 hasConcept C86803240 @default.
• W2180284899 hasConceptScore W2180284899C104317684 @default.

• next