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- W2180456423 abstract "Abstract The redclaw crayfish, Cherax quadricarinatus, is of commercially important interest, but its digestive capabilities are not yet completely understood. The understanding of how its digestive enzymes operate can help in the formulation of better diets for this species. A range of digestive enzymes (proteases, carbohydrases, and lipase) was found in the midgut gland and gastric fluid, with activities generally higher in the gastric fluid. Total protease showed maximal activity at pH 7.0–7.5 in both midgut gland and gastric fluid and was inhibited by 1.5 mM CuCl2 and 10 mM phenylmethylsulfonyl fluoride (PMSF). On the other hand, protease was activated by 1.5 mM CaCl2. Trypsin-like activity showed pH optima at 7.0 in both the midgut gland and the gastric fluid and was activated by 1.5 mM CaCl2, FeSO4, and Na2SO4. Chymotrypsin-like activity and carboxypeptidase A-like activity showed higher activities in the gastric fluid. Carboxypeptidase B-like activity showed optima at pH 6.0 and 7.0 in both the mid..." @default.
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- W2180456423 date "2001-05-01" @default.
- W2180456423 modified "2023-09-27" @default.
- W2180456423 title "DIGESTIVE ENZYME ACTIVITIES IN THE ALIMENTARY TRACT OF REDCLAW CRAYFISH, CHERAX QUADRICARINATUS (DECAPODA: PARASTACIDAE)" @default.
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- W2180456423 doi "https://doi.org/10.1651/0278-0372(2001)021[0334:deaita]2.0.co;2" @default.
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