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- W2180579609 abstract "Hypoxanthine Phosphoribosyltransferase (HGPRT; EC 2.4.2.8) is a central enzyme in the purine recycling pathway of all protozoan parasites. Protozoan parasites cannot synthesize purine bases (DNA/RNA) which is essential for survival as lack of de-novo pathway. Thus its good target for drug design and discovery as inhibition leads to cessation of replication. PRTase (transferase enzyme) has common PRTase type I folding pattern domain for its activities. Genomic studies revealed the sequence pattern and identified highly conserved residues that catalyzed the reaction in protozoan parasites. A recombinant protein has 24 kDa molecular mass (rLdHGPRT) was cloned, expressed and purified for testing of guanosine monophosphate (GMP) analogous compounds in-vitro by spectroscopically to the rLdHGPRT, lysates protein and MTT assay on Leishmania donovani. The predicted inhibitors of different libraries were screen into FlexX. The reported inhibitors were tested in-vitro. The 2'-deoxyguanosine 5'-diphosphate (DGD) (IC50 value 12.5 μM) is two times more effective when compared to guanosine-5'-diphosphate sodium (GD). Interestingly, LdHGPRT complex has shown stable after 24 ns in molecular dynamics simulation with interacting amino acids are Glu125, Ile127, Lys87 and Val186. QSAR studies revealed the correlation between predicted and experimental values has shown R2 0.998. Concludes that inversely proportional to their docked score with activities." @default.
- W2180579609 created "2016-06-24" @default.
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- W2180579609 date "2016-02-01" @default.
- W2180579609 modified "2023-10-16" @default.
- W2180579609 title "Establishment of correlation between in-silico and in-vitro test analysis against Leishmania HGPRT to inhibitors" @default.
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- W2180579609 cites W1486489268 @default.
- W2180579609 cites W1607116754 @default.
- W2180579609 cites W1963542761 @default.
- W2180579609 cites W1967650497 @default.
- W2180579609 cites W1968652333 @default.
- W2180579609 cites W1968888907 @default.
- W2180579609 cites W1970327093 @default.
- W2180579609 cites W1972789197 @default.
- W2180579609 cites W1977848104 @default.
- W2180579609 cites W1979606430 @default.
- W2180579609 cites W1981899562 @default.
- W2180579609 cites W1982471331 @default.
- W2180579609 cites W1983096703 @default.
- W2180579609 cites W1983304433 @default.
- W2180579609 cites W1984021058 @default.
- W2180579609 cites W1985240874 @default.
- W2180579609 cites W1988842403 @default.
- W2180579609 cites W1994016728 @default.
- W2180579609 cites W1994039834 @default.
- W2180579609 cites W1994251249 @default.
- W2180579609 cites W1995017064 @default.
- W2180579609 cites W1995789505 @default.
- W2180579609 cites W2008263090 @default.
- W2180579609 cites W2012969029 @default.
- W2180579609 cites W2014974078 @default.
- W2180579609 cites W2016313984 @default.
- W2180579609 cites W2019727879 @default.
- W2180579609 cites W2025990422 @default.
- W2180579609 cites W2028531632 @default.
- W2180579609 cites W2039763371 @default.
- W2180579609 cites W2045932791 @default.
- W2180579609 cites W2050456292 @default.
- W2180579609 cites W2051852689 @default.
- W2180579609 cites W2053663394 @default.
- W2180579609 cites W2055043387 @default.
- W2180579609 cites W2059775525 @default.
- W2180579609 cites W2061079400 @default.
- W2180579609 cites W2063982195 @default.
- W2180579609 cites W2066549591 @default.
- W2180579609 cites W2072863297 @default.
- W2180579609 cites W2074085078 @default.
- W2180579609 cites W2074796439 @default.
- W2180579609 cites W2079092300 @default.
- W2180579609 cites W2080603087 @default.
- W2180579609 cites W2081912151 @default.
- W2180579609 cites W2087661061 @default.
- W2180579609 cites W2094856731 @default.
- W2180579609 cites W2106810308 @default.
- W2180579609 cites W2106882534 @default.
- W2180579609 cites W2127553917 @default.
- W2180579609 cites W2130479394 @default.
- W2180579609 cites W2131835194 @default.
- W2180579609 cites W2133644793 @default.
- W2180579609 cites W2152873608 @default.
- W2180579609 cites W2152974563 @default.
- W2180579609 cites W2155283295 @default.
- W2180579609 cites W2158714788 @default.
- W2180579609 cites W2159278561 @default.
- W2180579609 cites W2159400556 @default.
- W2180579609 cites W2171268876 @default.
- W2180579609 cites W2319644338 @default.
- W2180579609 cites W2327801645 @default.
- W2180579609 cites W4293247451 @default.
- W2180579609 cites W992556388 @default.
- W2180579609 doi "https://doi.org/10.1016/j.ijbiomac.2015.11.051" @default.
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