FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2181356664> ?p ?o ?g. }

• W2181356664 endingPage "2395" @default.
• W2181356664 startingPage "2386" @default.
• W2181356664 abstract "Pseudomonas aeruginosa is an opportunistic human pathogen for which new antimicrobial drug options are urgently sought. P. aeruginosa disulfide-bond protein A1 (PaDsbA1) plays a pivotal role in catalyzing the oxidative folding of multiple virulence proteins and as such holds great promise as a drug target. As part of a fragment-based lead discovery approach to PaDsbA1 inhibitor development, the identification of a crystal form of PaDsbA1 that was more suitable for fragment-soaking experiments was sought. A previously identified crystallization condition for this protein was unsuitable, as in this crystal form of PaDsbA1 the active-site surface loops are engaged in the crystal packing, occluding access to the target site. A single residue involved in crystal-packing interactions was substituted with an amino acid commonly found at this position in closely related enzymes, and this variant was successfully used to generate a new crystal form of PaDsbA1 in which the active-site surface is more accessible for soaking experiments. The PaDsbA1 variant displays identical redox character and in vitro activity to wild-type PaDsbA1 and is structurally highly similar. Two crystal structures of the PaDsbA1 variant were determined in complex with small molecules bound to the protein active site. These small molecules (MES, glycerol and ethylene glycol) were derived from the crystallization or cryoprotectant solutions and provide a proof of principle that the reported crystal form will be amenable to co-crystallization and soaking with small molecules designed to target the protein active-site surface." @default.
• W2181356664 created "2016-06-24" @default.
• W2181356664 creator A5011456426 @default.
• W2181356664 creator A5021672406 @default.
• W2181356664 creator A5028427338 @default.
• W2181356664 creator A5040201335 @default.
• W2181356664 creator A5040684603 @default.
• W2181356664 creator A5041973018 @default.
• W2181356664 creator A5048577006 @default.
• W2181356664 date "2015-11-26" @default.
• W2181356664 modified "2023-09-26" @default.
• W2181356664 title "Sent packing: protein engineering generates a new crystal form of<i>Pseudomonas aeruginosa</i>DsbA1 with increased catalytic surface accessibility" @default.
• W2181356664 cites W1788190869 @default.
• W2181356664 cites W1793924104 @default.
• W2181356664 cites W1971048984 @default.
• W2181356664 cites W2002920425 @default.
• W2181356664 cites W2010300361 @default.
• W2181356664 cites W2035503835 @default.
• W2181356664 cites W2035703715 @default.
• W2181356664 cites W2036357948 @default.
• W2181356664 cites W2058647917 @default.
• W2181356664 cites W2092908442 @default.
• W2181356664 cites W2098167941 @default.
• W2181356664 cites W2105075420 @default.
• W2181356664 cites W2108921801 @default.
• W2181356664 cites W2110808180 @default.
• W2181356664 cites W2113442929 @default.
• W2181356664 cites W2115634065 @default.
• W2181356664 cites W2122529728 @default.
• W2181356664 cites W2122994276 @default.
• W2181356664 cites W2136390204 @default.
• W2181356664 cites W2139451307 @default.
• W2181356664 cites W2144081223 @default.
• W2181356664 cites W2147949826 @default.
• W2181356664 cites W2151174719 @default.
• W2181356664 cites W2154197653 @default.
• W2181356664 cites W2154714625 @default.
• W2181356664 cites W2163341755 @default.
• W2181356664 cites W2165045067 @default.
• W2181356664 cites W2168974130 @default.
• W2181356664 cites W2173920250 @default.
• W2181356664 cites W4210675630 @default.
• W2181356664 doi "https://doi.org/10.1107/s1399004715018519" @default.
• W2181356664 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4667283" @default.
• W2181356664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26627647" @default.
• W2181356664 hasPublicationYear "2015" @default.
• W2181356664 type Work @default.
• W2181356664 sameAs 2181356664 @default.
• W2181356664 citedByCount "5" @default.
• W2181356664 countsByYear W21813566642017 @default.
• W2181356664 countsByYear W21813566642018 @default.
• W2181356664 countsByYear W21813566642021 @default.
• W2181356664 countsByYear W21813566642023 @default.
• W2181356664 crossrefType "journal-article" @default.
• W2181356664 hasAuthorship W2181356664A5011456426 @default.
• W2181356664 hasAuthorship W2181356664A5021672406 @default.
• W2181356664 hasAuthorship W2181356664A5028427338 @default.
• W2181356664 hasAuthorship W2181356664A5040201335 @default.
• W2181356664 hasAuthorship W2181356664A5040684603 @default.
• W2181356664 hasAuthorship W2181356664A5041973018 @default.
• W2181356664 hasAuthorship W2181356664A5048577006 @default.
• W2181356664 hasBestOaLocation W21813566641 @default.
• W2181356664 hasConcept C115624301 @default.
• W2181356664 hasConcept C153064111 @default.
• W2181356664 hasConcept C161624437 @default.
• W2181356664 hasConcept C178790620 @default.
• W2181356664 hasConcept C181199279 @default.
• W2181356664 hasConcept C185592680 @default.
• W2181356664 hasConcept C199360897 @default.
• W2181356664 hasConcept C203036418 @default.
• W2181356664 hasConcept C21951064 @default.
• W2181356664 hasConcept C2777637488 @default.
• W2181356664 hasConcept C2781285689 @default.
• W2181356664 hasConcept C2781338088 @default.
• W2181356664 hasConcept C32909587 @default.
• W2181356664 hasConcept C41008148 @default.
• W2181356664 hasConcept C41183919 @default.
• W2181356664 hasConcept C523546767 @default.
• W2181356664 hasConcept C54355233 @default.
• W2181356664 hasConcept C55493867 @default.
• W2181356664 hasConcept C71240020 @default.
• W2181356664 hasConcept C8010536 @default.
• W2181356664 hasConcept C86803240 @default.
• W2181356664 hasConceptScore W2181356664C115624301 @default.
• W2181356664 hasConceptScore W2181356664C153064111 @default.
• W2181356664 hasConceptScore W2181356664C161624437 @default.
• W2181356664 hasConceptScore W2181356664C178790620 @default.
• W2181356664 hasConceptScore W2181356664C181199279 @default.
• W2181356664 hasConceptScore W2181356664C185592680 @default.
• W2181356664 hasConceptScore W2181356664C199360897 @default.
• W2181356664 hasConceptScore W2181356664C203036418 @default.
• W2181356664 hasConceptScore W2181356664C21951064 @default.
• W2181356664 hasConceptScore W2181356664C2777637488 @default.
• W2181356664 hasConceptScore W2181356664C2781285689 @default.
• W2181356664 hasConceptScore W2181356664C2781338088 @default.
• W2181356664 hasConceptScore W2181356664C32909587 @default.
• W2181356664 hasConceptScore W2181356664C41008148 @default.
• W2181356664 hasConceptScore W2181356664C41183919 @default.

• next