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- W2181382811 abstract "Synthesis of the gemcitabine-loaded PLGA nanoparticles: a water-in-oil emulsion was prepared by mixing appropriate volumes of a gemcitabine aqueous solution with 1 mL of a 1 % (w/v) PLGA and 0.5 % (w/v) Ethocel solution in ethyl acetate. This emulsion was used as focused fluid in a simple flow focusing nozzle [Avant 2 (D = 100 μm), Ingeniatrics Tecnologias S.L., Spain] fixed at different flow rates (table 1). Distilled water was used as focusing fluid. PLGA nanoparticles were formed into a 0.3 % (w/v) PVA solution. The ethyl acetate content was reduced to a very minimum by using a rotary evaporator. Then, they were freeze-dried and stored at ≈ 4 oC until use. Characterization methods: the mean particle size and particle size distributions of gemcitabineloaded PLGA nanoparticles were measured at ≈ 25 oC by laser scattering (Partica LA-950V2, Horiba). Gemcitabine loading was determined by reverse phase-high performance liquid chromatography (RP-HPLC) (Hitachi LaChrom® (D-7000) Series HPLC system). Gemcitabine content was expressed in terms of gemcitabine entrapment efficiency (EE, %) [2]. The production performance (%) was also determined [6]. In order to establish the variations in particle size, a statistical analysis was performed by the use of the Student’s t-test. Values with p < 0.05 and p < 0.01 were considered as significantly different." @default.
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- W2181382811 date "2010-01-01" @default.
- W2181382811 modified "2023-10-16" @default.
- W2181382811 title "Engineering of gemcitabine-loaded poly(D,L-lactide-co-glycolide) nanoparticles by flow focusing for cancer treatment" @default.
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