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- W2181392322 abstract "A key challenge in mammalian biology is to understand how rates of transcription and mRNA degradation jointly shape cellular gene expression. Powerful techniques have been developed for measuring these rates either genome-wide or at the single-molecule level, however these techniques are not applicable to assessment of cells within their native tissue microenvironment. Here we describe a technique based on single molecule Fluorescence in-situ Hybridization (smFISH) to measure transcription and degradation rates in intact mammalian tissues. The technique is based on dual-color libraries targeting the introns and exons of the genes of interest, enabling visualization and quantification of both nascent and mature mRNA. We present a software, TransQuant, that facilitates quantifying these rates from smFISH images. Our approach enables assessment of both transcription and degradation rates of any gene of interest while controlling for the inherent heterogeneity of intact tissues." @default.
- W2181392322 created "2016-06-24" @default.
- W2181392322 creator A5042875880 @default.
- W2181392322 creator A5043816772 @default.
- W2181392322 date "2016-04-01" @default.
- W2181392322 modified "2023-10-18" @default.
- W2181392322 title "Single molecule approaches for quantifying transcription and degradation rates in intact mammalian tissues" @default.
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- W2181392322 doi "https://doi.org/10.1016/j.ymeth.2015.11.015" @default.
- W2181392322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26611432" @default.
- W2181392322 hasPublicationYear "2016" @default.
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