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- W2181393501 abstract "Contamination control and elimination have been a major concern in cell culture laboratories for over 100 years. Though other technologies have been developed over this period for instruments, devices, and textiles, dry heat sterilization has emerged as the most useful contamination elimination technology for equipment chamber surfaces. A dry heat sterilization cycle has now been validated for the Thermo Scientific Steri-Cycle® CO2 Incubators. This sterilization cycle readily eliminates the vegetative microorganisms, as well as persistent fungal spores, of concern in cell culture laboratories. Cycle time is based on validation procedures using commercially available spore suspensions calibrated for dry heat resistance. The spores were inoculated onto chamber wall, door, and gasket materials. The inoculated materials were exposed at the minimum temperature (the coolest, and therefore least lethal) observed in the incubator as determined by extensive mapping studies. The validated cycle time was based on the longest average time required to achieve a six-log reduction of the most resistant spore batch tested. This exposure time was then more than doubled to establish a sterilization cycle that exceeds the requirements for medical instruments." @default.
- W2181393501 created "2016-06-24" @default.
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- W2181393501 date "2007-01-01" @default.
- W2181393501 modified "2023-09-27" @default.
- W2181393501 title "Effective Heat Sterilization in CO 2 Incubators" @default.
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