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• W2181904917 abstract "Background: Methicillin-resistant Staphylococci; both methicillin resistant S. aureus (MRSA) and methicillin resistant coagulase negative Staphylococci (MRCNS) have a worldwide distribution and are important causes of clinical and epidemiological problems. The aim of this study was to evaluate the usefulness of some phenotypic methods for detection of methicillin resistant Staphylococci in clinical laboratories; cefoxitin disc diffusion (CDD), oxacillin resistance screening agar base (ORSAB) and oxacillin E test, in reference to mecA gene based real-time PCR. Also to study the antibiotic resistance pattern of the methicillin resistant Staphylococci isolates. Materials and methods: A total of 95 clinical isolates of Staphylococci were tested for methicillin resistance by CDD test, ORSAB and oxacillin E-test and were compared to mecA based real time PCR as reference method. Results: MecA gene was detected by PCR in 48/95 (51%) of all Staphylococci isolates; 28/57 (49%) in S. aureus and 20/38 (53%) in CNS isolates. CCD test showed 100% sensitivity and 98% specificity for detection of MRSA and MRCNS. ORSAB and Oxacillin E test had 94% and 90% sensitivity and 96% and 98% specificity respectively. In our study, MRSA isolates were resistant to ciprofloxacin-78%, erythromycin-74%, clindamycin71%, Gentamicin-70%, amikacin64%, azithromycin-63%, doxycyclin-60%, levofloxacin and linezolid-48%, trimethoprim-sulfamethoxazole-29% and vancomycin-0%. Conclusion: Cefoxitin disc diffusion can be used as a reliable conventional, simple and cheap alternative to PCR for detection of MRSA and MRCNS in minimal resources circumstances. It can also detect other mechanisms of resistance other than mecA. Additional confirmatory test is needed with oxacillin screening agar and oxacillin E-test for detection of hetero-resistant strains to methicillin. [Samia A. Girgis, Howida E. Gomaa, Nevine E. Saad and Mai M. Salem. A Comparative Study for Detection of Methicillin Resistance Staphylococci by Polymerase Chain Reaction and Phenotypic Methods. Life Sci J 2013; 10(4):3711-3718] (ISSN: 1097-8135). http://www.lifesciencesite.com. 499" @default.
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• W2181904917 date "2013-01-01" @default.
• W2181904917 modified "2023-09-25" @default.
• W2181904917 title "A Comparative Study for Detection of Methicillin Resistance Staphylococci by Polymerase Chain Reaction and Phenotypic Methods" @default.
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