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- W2182067660 abstract "Witches’ broom, or Sphaeropsis gall, is an important disease of some holly species and cultivars in central and south Florida landscapes and nurseries. The fungal pathogen, S. tumefaciens, was isolated from symptomatic holly trees in Gainesville and Apopka landscapes. Agar plugs containing hyphae of the pathogen were used to inoculate pruning wounds made on 15 commercially available cultivars. A growing tip, secondary branch, and the main stem of each plant were inoculated in July 2005. By mid-August symptoms were apparent. Mean separations of lesion length showed statistically significant differences between cultivars. No cultivar was immune, and species of Ilex found most susceptible were I. glabra ‘Nigra’, I. X ‘Mary Nell’, I. crenata ‘Compacta’, and I. crenata ‘Helleri’. Objectives, Materials, and Methods: The objective of this project was to quantify differences in the susceptibility of holly cultivars important to the Florida nursery and landscape industries. In order to accomplish this objective, virulent isolates of the pathogen were collected, cultured, and stored. Holly cultivars important to the industry were identified and obtained. An inoculation method was developed that would mimic natural inoculation in a landscape or nursery situation. A method for rating disease and quantifying differences in susceptibility was developed. Diseased samples exhibiting galling, blighting, and witches’ broom symptoms were collected from holly plants in Gainesville and Apopka landscapes. Affected galls and shoots were surface sterilized in a 10% household bleach solution and plated onto potato dextrose agar amended with antibiotics. Colonies with morphology similar to S. tumefaciens were transferred and stored for future use. Nine isolates were collected. Isolate UF05161 was selected for this trial and was increased on V8 juice agar." @default.
- W2182067660 created "2016-06-24" @default.
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- W2182067660 date "2005-01-01" @default.
- W2182067660 modified "2023-09-24" @default.
- W2182067660 title "Differences in Susceptibility of Ornamental Holly Cultivars to Sphaeropsis tumefaciens" @default.
- W2182067660 hasPublicationYear "2005" @default.
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