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- W2182079076 abstract "The development of DNA microarray technology for high-throughput gene synthesis is hampered by poor sequence quality and inefficient gene assembly. We developed a programmable microchip using thermoplastic cyclic olefin copolymer (COC) functionalized with hydrophilic SiO2 thin film arrays, creating physically isolated picolitter reactors that constrain liquid via differential wettability. These functionalized SiO2-COC microchips exhibit enhanced droplet confinement and reduced edge-effect during in situ DNA synthesis, producing high-quality oligonucleotide arrays. Further, we demonstrated accurate and effective on-chip gene assembly from complex and heterogeneous microarray oligonucleotides by integrated amplification and assembly of sub-arrays in physically-isolated reaction chambers." @default.
- W2182079076 created "2016-06-24" @default.
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- W2182079076 date "2013-01-01" @default.
- W2182079076 modified "2023-09-22" @default.
- W2182079076 title "PATTERNING AND FUNCTIONALIZATION OF THERMOPLASTIC MICROCHIP FOR AUTOMATED HIGH-THROUGHPUT MICROARRAY GENE SYNTHESIS" @default.
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