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- W2182083732 abstract "Prevention of Infectious Bursal Disease (IBD) is very crucial in poultry production because the disease makes the birds susceptible to other diseases through immunosuppression. Thus prevention is preferred to treatment in its control. Prevention of the disease has been mainly achieved by active vaccination of layers to facilitate transfer of IBDV maternal antibodies (Mab) to chicks via the eggs, thereby protecting chicks during the first few weeks before active vaccination. But most active vaccination of chicks against the virus had resulted in failure due to interference by maternal antibodies and inability to determine the crucial time for active vaccination of chicks without interference by maternal antibodies. The methods of Enzyme Linked Immunosorbent Assay (ELISA) by titration, virus neutralization or Agar Gel Precipitation Tests commonly used are unreliable as far as generating daily serological data was concerned in collaboration with the Department of Animal Science, Guangxi University, Guangxi Province, Peoples’ Republic of China, as these are long, tedious less sensitive. ELISA in single dilution has the inherent capacity of overcoming this limitation and therefore was employed in monitoring maternal antibodies to determine the right day to vaccinate chicks without maternal antibody interference. Thus using ELISA in single dilution, a batch of randomly selected 30 chicks and blood samples from 25 vaccinated hens were obtained from a breeder farm, monitored for maternal antibody depletion in comparison to the titres of the hens for four weeks. ELISA in single dilution more specifically, rapidly and accurately measured the antibody titres of the birds from day old to twenty four days. A correlation coefficient of 0.83 (P ≤0.05) was obtained by linear regression of ELISA titres and positive/negative ratios. The average Mab of the offspring ( 5" @default.
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- W2182083732 date "2013-01-01" @default.
- W2182083732 modified "2023-09-27" @default.
- W2182083732 title "Appropriate time of vaccination against infectious bursal disease virus in layer chicks by Elisa in single dilution" @default.
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